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首页> 外文期刊>RSC Advances >Dual-stabilizer-capped CdSe quantum dots for 'Off-On' electrochemiluminescence biosensing of thrombin by target-triggered multiple amplification
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Dual-stabilizer-capped CdSe quantum dots for 'Off-On' electrochemiluminescence biosensing of thrombin by target-triggered multiple amplification

机译：双稳压器封端的CDSE量子点，用于“OFF-on”电化学发光通过目标触发多扩增凝血酶的生物沉积

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摘要

In this work, new dual-stabilizer-capped CdSe quantum dots (QDs) with unique electrochemiluminescence (ECL) were prepared, and used to design a promising "off-on" ECL biosensor for highly sensitive assay of thrombin by target-triggered multiple amplification for the first time. New poly(diallyldimethylammonium chloride) (PDDA)-graphene oxide (GO) nanocomposites with good electroconductibility and much surface area was used to assemble a large number of CdSe QDs on the electrode, showing excellent ECL and stability. The ECL of CdSe QDs was efficiently quenched by gold nanoparticles (off state), the presence of target thrombin resulted in the generation of numerous DNA sequences (t1) by using multiple DNA cycle amplification techniques. Subsequently, the specific hybridization of abundant t1 to the quencher probe caused significantly amplified ECL enhancement (on state), which was used for highly sensitive detection of thrombin. More importantly, the biosensor with the dual-stabilizer capped CdSe QDs demonstrated highly intense ECL emission, and the DNA devices by the cleavage reaction were cycled for multiple rounds, which greatly amplified the ECL signal and much improved the detection sensitivity. The developed assay protocol also shows high selectivity for the target and can be performed in homogeneous solutions. These distinct advantages make it a potential platform for detecting various types of biomolecules. This flexible biosensing system exhibits excellent performance in real human serum assay, which has wider potential applications than other ECL reagent-based system.
机译：在这项工作中，制备了具有独特电综合发光（ECL）的新的双稳压器封端的CDSE量子点（QDS），并用于通过目标触发的多扩增设计有希望的“脱机”ECOR生物传感器，用于凝血酶的高敏感测定首次。使用具有良好导电性和大部分表面积的新聚（二烯丙基甲基氯化铵）（PDDA）型氧化物（GO）纳米复合材料用于在电极上组装大量CdSe QD，显示出优异的ECL和稳定性。通过金纳米颗粒（OFF状态）有效地淬灭CDSEQDS的ECL，通过使用多种DNA循环扩增技术产生靶凝血酶的存在导致多种DNA序列（T1）产生。随后，丰富的T1对猝灭剂探针的比杂交引起显着扩增的ECL增强（ON状态），其用于凝血酶的高敏感性检测。更重要的是，具有双稳定剂的生物传感器封端Cdse QDS表现出高度激烈的ECL发射，并且通过切割反应的DNA装置循环多个圆形，这大大放大了ECL信号并大大提高了检测灵敏度。开发的测定方案还显示了对目标的高选择性，并且可以在均匀的溶液中进行。这些独特的优点使其成为检测各种类型的生物分子的潜在平台。这种柔性生物传感系统在真正的人体血清测定中表现出优异的性能，其具有比其他基于ECL试剂的系统更宽的潜在应用。

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  • 来源
    《RSC Advances》 |2016年第3期|共7页
  • 作者

    Jie Guifen; Chen Kai; Wang Xiaochun; Lu Zhengkun;

  • 作者单位

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Coll Chem &

    Mol Engn Minist Educ Qingdao 266042 Peoples R China;

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Coll Chem &

    Mol Engn Minist Educ Qingdao 266042 Peoples R China;

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Coll Chem &

    Mol Engn Minist Educ Qingdao 266042 Peoples R China;

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Coll Chem &

    Mol Engn Minist Educ Qingdao 266042 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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