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Development of functionalized multi-walled carbon nanotube-based polysaccharide-hydroxyapatite scaffolds for bone tissue engineering

机译：骨组织工程官能化多壁碳纳米管多糖 - 羟基磷灰石支架的研制

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摘要

New tricomponent composite scaffolds with high porosity (82.82% for oxidized carbon nanotube (fMWCNT)-gellan-hydroxyapatite (HAP) and 91.76% for fMWCNT-amylopectin-HAP) were prepared by a freeze drying method and characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FE-SEM). Further, their ability to promote cell proliferation of the MG 63 cell line for bone regeneration was studied. In vitro MTT assays with the MG 63 cell line demonstrated better cell proliferation on both the fMWCNT-gellan-HAP and fMWCNT-amylopectin-HAP scaffolds. The cell adhesion of the MG 63 cells on the scaffolds after four days was confirmed by fluorescence imaging using Hoechst and Acridine Orange dye staining. Enhanced mineralization over time was observed in the alkaline phosphatase (ALP) activity. A higher compressive strength (222.60 +/- 8.4 kPa) was observed for fMWCNT-gellan-HAP, when compared to fMWCNT-amylopectin-HAP (57.32 +/- 2.4 kPa). This may be due to the lower porosity of fMWCNT-gellan-HAP. These results ascertained that the prepared tricomponent scaffolds can be used as promising materials in bone tissue engineering.
机译：具有高孔隙率（对于氧化处理碳纳米管（fMWCNT）-gellan羟基磷灰石（HAP）和91.76％对fMWCNT-支链淀粉-HAP 82.82％）新三组分复合支架被冷冻干燥方法制备和表征通过傅里叶变换红外光谱（FT -IR），X射线衍射（XRD），热重量分析（TGA）和场发射扫描电子显微镜（FE-SEM）。另外，其促进骨再生的MG 63细胞系的细胞增殖的能力进行了研究。体外MTT测定与MG 63细胞系表现出对两者fMWCNT-吉兰-HAP和fMWCNT-支链淀粉-HAP支架更好的细胞增殖。上四天后支架的MG 63细胞的细胞粘附通过使用Hoechst的和吖啶橙染料染色荧光成像确认。在碱性磷酸酶（ALP）活性，观察到随着时间的推移提高矿化。观察到fMWCNT-吉兰-HAP较高抗压强度（222.60 +/- 8.4千帕），相比fMWCNT-支链淀粉-HAP（57.32 +/- 2.4千帕）时。这可能是由于fMWCNT-吉兰-HAP的较低的孔隙率。这些结果确认了所制备的三组分支架可作为有希望的骨组织工程材料。

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  • 来源
    《RSC Advances》 |2016年第85期|共9页
  • 作者

    Rajesh R.; Ravichandran Y. Dominic; Reddy M. Jeevan Kumar; Ryu Sung Hun; Shanmugharaj A. M.;

  • 作者单位

    Karpagam Coll Engn Dept Sci &

    Humanities Coimbatore 641032 Tamil Nadu India;

    Karpagam Coll Engn Dept Sci &

    Humanities Coimbatore 641032 Tamil Nadu India;

    Kyung Hee Univ Dept Chem Engn Yongin 446701 Kyunggi Do South Korea;

    Kyung Hee Univ Dept Chem Engn Yongin 446701 Kyunggi Do South Korea;

    Kyung Hee Univ Dept Chem Engn Yongin 446701 Kyunggi Do South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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