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Enzymatic manipulation of a DNA-mediated ensemble for sensitive fluorescence detection of glucose

机译：酶促操纵DNA介导的葡萄糖敏感荧光检测的组合

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摘要

Recently small molecules with fluorescence properties sensitive to DNA have been identified as efficient fluorescent probes. Herein, controllable turn off/on fluorescent sensors for label-free detection of glucose have been successfully developed by designing different DNA/ligand-based ensembles and using the enzyme-catalyzed Fenton reaction. The sensing approach is essentially based on enzymatic manipulation of a DNA-mediated ensemble through cascade reactions, including oxidation of glucose by oxygen to yield hydrogen peroxide, the Fenton reaction of H2O2 resulting in the formation of aggressive hydroxyl radicals ((OH)-O-center dot), and then the oxidative cleavage of ssDNA with (OH)-O-center dot. Interestingly, the fact that the properties of DNA-mediated ensembles are highly dependent on the nature of ligands opens the opportunity to modulate the response patterns of fluorescent probes to glucose simply by choosing some appropriate ligands. Additionally, the protocol shows excellent selectivity, and visual detection is possible with the aid of an UV transilluminator. Moreover, this method can also be extended as a novel analytical platform for other reactions that involve the H2O2 concentration evolution.
机译：最近，具有对DNA敏感的荧光特性的小分子已被鉴定为有效的荧光探针。这里，通过设计不同的DNA /配体基集合并使用酶催化的芬顿反应，已经成功开发了可控制的无标记检测葡萄糖的荧光传感器。传感方法基本上基于通过级联反应的DNA介导的集合的酶促操作，包括通过氧气氧化葡萄糖以产生过氧化氢，H2O2的芬顿反应导致形成侵袭性羟基（（OH）-O-中心点），然后用（OH）-O中心点的SSDNA氧化切割。有趣的是，DNA介导的合奏的性质高度依赖于配体的性质，通过选择一些合适的配体来调节荧光探针对葡萄糖的响应模式的机会。另外，该方案显示出优异的选择性，借助UV传输仪可以进行视觉检测。此外，该方法也可以作为一种新的分析平台延伸，用于其他涉及H 2 O 2浓度进化的反应。

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  • 来源
    《RSC Advances》 |2016年第39期|共6页
  • 作者

    Jiang Yaoping; Luo Wenhao; Wang Xiaopei; Lin Youhui; Liu Xiang Yang;

  • 作者单位

    Xiamen Univ Res Inst Biomimet &

    Soft Matter Dept Phys Fujian Prov Key Lab Soft Funct Mat Res Xiamen 361005 Peoples R China;

    Xiamen Univ Res Inst Biomimet &

    Soft Matter Dept Phys Fujian Prov Key Lab Soft Funct Mat Res Xiamen 361005 Peoples R China;

    Xiamen Univ Res Inst Biomimet &

    Soft Matter Dept Phys Fujian Prov Key Lab Soft Funct Mat Res Xiamen 361005 Peoples R China;

    Xiamen Univ Res Inst Biomimet &

    Soft Matter Dept Phys Fujian Prov Key Lab Soft Funct Mat Res Xiamen 361005 Peoples R China;

    Xiamen Univ Res Inst Biomimet &

    Soft Matter Dept Phys Fujian Prov Key Lab Soft Funct Mat Res Xiamen 361005 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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