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首页> 外文期刊>RSC Advances >Preparation and characterization of a Combi-CLEAs from pectinases and cellulases: a potential biocatalyst for grape juice clarification
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Preparation and characterization of a Combi-CLEAs from pectinases and cellulases: a potential biocatalyst for grape juice clarification

机译：来自果胶酶和纤维素酶的组合和表征的组合和表征：葡萄汁的潜在生物催化剂澄清

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摘要

Combined cross-linked enzyme aggregates (combi-CLEAs) are a novel prospect for immobilization of a mixture of enzymes and the present study addresses the preparation, characterization and application of pectinases-cellulases combi-CLEAs for grape juice clarification. Initially, 8 enzymatic preparations were tested for turbidity reduction in grape juice and Rohapect 10L provided the best results (around 50% in 1 h) being selected for CLEA preparation. The optimization of combi-CLEAs, was performed using a central composite design (CCD) and response surface methodology (RSM) varying the glutaraldehyde concentration and reaction time, using isopropanol as the precipitant agent. The best conditions for the Rohapect 10L CLEA preparation was 110 mM of glutaraldehyde and 2 h. Bovine serum albumin (BSA) was used as a feeder and improved the volumetric activity, recovered activity and thermal stability. Combi-CLEAs-BSA prepared using 0.4 mg mL(-1) of the enzyme mixture and 2.4 mg mL(-1) of BSA presented an activity of 14 U mL(-1), 18% of recovered activity and 3-times more thermal stability compared to soluble enzymes. The combi-CLEAs and combi-CLEAs-BSA were tested in repeated batches, being reused for 4 and 6 cycles, respectively, keeping 100% of the initial activity. The combi-CLEAs and combi-CLEAs-BSA appear to be suitable alternatives of immobilized biocatalyst for the clarification of grape juices.
机译：结合的交联酶聚集体（COMBI-CLEA）是固定酶混合物的新展前，并本研究解决了果胶酸纤维酶的制备，表征和应用葡萄汁澄清。最初，测试8种酶制剂，用于葡萄汁中的浊度减少，罗布分析10L提供了最佳结果（约50％以上1小时）用于CLEA制备。使用中央复合设计（CCD）和反应表面方法（RSM）进行组合CLA的优化，并使用异丙醇作为沉淀剂，改变戊二醛浓度和反应时间。 Rohapect 10L CLEA制剂的最佳条件为110毫米戊二醛和2小时。牛血清白蛋白（BSA）用作进料器并改善体积活性，回收活性和热稳定性。使用0.4mg ml（-1）的酶混合物和2.4mg ml（-1）Bsa制备的Combi-cla-bsa呈现了14u ml（-1），18％回收活性的活性和3倍与可溶性酶相比热稳定性。在重复批次中测试Combi-ClA基和Combi-ClE-BSA，分别在4和6个循环中重复使用，保持100％的初始活性。 Combi-CleA和Combi-Cle-BSA似乎是固定化生物催化剂的合适替代品，用于澄清葡萄汁。

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  • 来源
    《RSC Advances》 |2016年第32期|共10页
  • 作者

    Dal Magro Lucas; Hertz Plinho F.; Fernandez-Lafuente Roberto; Klein Manuela P.; Rodrigues Rafael C.;

  • 作者单位

    Univ Fed Rio Grande do Sul Inst Food Sci &

    Technol Biotechnol Bioproc &

    Biocatalysis Grp Av Bento Goncalves 9500 POB 15090 BR-91501970 Porto Alegre RS Brazil;

    Univ Fed Rio Grande do Sul Inst Food Sci &

    Technol Biotechnol Bioproc &

    Biocatalysis Grp Av Bento Goncalves 9500 POB 15090 BR-91501970 Porto Alegre RS Brazil;

    ICP CSIC Dept Biocatalisis Campus UAM CSIC Madrid 28049 Spain;

    Univ Fed Rio Grande do Sul Inst Food Sci &

    Technol Biotechnol Bioproc &

    Biocatalysis Grp Av Bento Goncalves 9500 POB 15090 BR-91501970 Porto Alegre RS Brazil;

    Univ Fed Rio Grande do Sul Inst Food Sci &

    Technol Biotechnol Bioproc &

    Biocatalysis Grp Av Bento Goncalves 9500 POB 15090 BR-91501970 Porto Alegre RS Brazil;

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  • 正文语种 eng
  • 中图分类 化学;
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