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首页> 外文期刊>RSC Advances >Interactions of cucurbit[6,7]urils with human serum albumin and their effects on zaltoprofen transportation
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Interactions of cucurbit[6,7]urils with human serum albumin and their effects on zaltoprofen transportation

机译：葫芦（6,7]尿嘧啶与人血清白蛋白的相互作用及其对ZaltObrofen运输的影响

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摘要

Cucurbit[6,7]urils (CB[6,7]s) have been extensively investigated because of their characteristic inclusion ability. To enhance our understanding of the behaviors of CB[6,7]s in biological environments and explore their effects on drug transportation, multi-spectroscopic and molecular docking methods were applied to examine the binding mechanism of CB[6,7]s with human serum albumin (HSA) and their interference on the HSA-zaltoprofen (ZPF) binding system. H-1 NMR spectra revealed the complexes that formed between CB[6,7]s and HSA, as well as between ZPF and HSA. The fluorescence of HSA was quenched by ZPF through a combined static and dynamic mechanism. The added CB[6,7]s did not significantly affect the energy transfer efficiency, but decreased the HSA-ZPF binding constant. The binding stoichiometry of ZPF with HSA was 1 : 1, and this value remained unchanged when CB[6,7]s were added. Circular dichroism spectroscopy, synchronous and 3D fluorescence spectroscopy demonstrated that CB[6,7]s slightly influenced the native secondary structure of HSA and its ZPF-induced unfolding. Further molecular docking suggested that CB[6,7]s preferred to bind to the HSA surface, and ZPF was primarily located in an L-shaped cavity in subdomain IB. Therefore, CB[6,7]s elicited minor damage to the structure of HSA and weak interferance with drug transportation.
机译：由于其特征包容能力，葫芦[6,7] URIL（CB [6,7]）已被广泛调查。为了提高我们对生物环境中CB [6,7] S的行为的理解，并探讨其对药物运输的影响，施用多光谱和分子对接方法以检查CB [6,7] S与人的结合机制血清白蛋白（HSA）及其对HSA-ZaltOroprofen（ZPF）结合系统的干扰。 H-1 NMR光谱揭示了在CB [6,7] S和HSA之间形成的复合物，以及ZPF和HSA。通过组合的静态和动态机制通过ZPF淬灭HSA的荧光。添加的CB [6,7] S没有显着影响能量转移效率，但降低了HSA-ZPF结合常数。 ZPF与HSA的结合化学计量为1：1，当加入CB [6,7]时，该值保持不变。圆形二色谱，同步和3D荧光光谱表明CB [6,7] S略微影响了HSA的天然二级结构及其ZPF诱导的展开。进一步的分子对接表明CB [6,7]优选与HSA表面结合，ZPF主要位于子域IB中的L形腔中。因此，CB [6,7]对HSA结构的轻微损害和药物运输弱干扰。

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  • 来源
    《RSC Advances》 |2016年第89期|共9页
  • 作者

    Li Shanshan; He Jiawei; Huang Yanmei; Wang Qing; Yang Hongqin; Xu Kailin; Li Hui;

  • 作者单位

    Sichuan Univ Coll Chem Engn Chengdu 610065 Peoples R China;

    Sichuan Univ Coll Chem Engn Chengdu 610065 Peoples R China;

    Sichuan Univ Coll Chem Engn Chengdu 610065 Peoples R China;

    Sichuan Univ Coll Chem Engn Chengdu 610065 Peoples R China;

    Sichuan Univ Coll Chem Engn Chengdu 610065 Peoples R China;

    Sichuan Univ Coll Chem Engn Chengdu 610065 Peoples R China;

    Sichuan Univ Coll Chem Engn Chengdu 610065 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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