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Unusual surface and solution behaviour of keratin polypeptides

机译：角蛋白多肽的异常表面和解决方案行为

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Keratins are filament proteins, but we report in this work that water-soluble keratin polypeptides hydrolyzed from wool could readily adsorb onto the surface of water and could thus be used as surface active biomaterials. Neutron reflection measurements with the help of deuterium labelling were used to determine the adsorbed amount and distribution of the polypeptide layers formed. It was found that the interfacial layers were comprised of two main regions, a dense top layer of 18-25 angstrom and a loose bottom layer of 25-30 angstrom. Half of the top dense layer was exposed to air with the remainder of the top layer and the diffuse bottom layer immersed in the aqueous solution. Both the volume fraction and the layer thickness increased with keratin solution concentration as did the adsorbed amount which was seen to plateau just above 2 mg m(-2) at approximately 0.1 g dm(-3) (2.1 mu M). Increase in [NaCl] led to reduced surface adsorption, accompanied with the thinning of the top layer. Cryo-TEM imaging revealed that the keratin aggregates had an ellipsoidal structure with radii ranging from 60 angstrom to 220 angstrom. The ellipsoidal shape was well supported by SANS, with the major radius of 140 angstrom and the minor radius of 60 angstrom. With increasing [NaCl], the ellipsoids became thinner but longer, a feature consistent with the observed trend from surface adsorbed layer. This unusual behaviour could be explained by the electrostatic screening effect. As the salt concentration increased, the polypeptide chains became stiffer and more readily aligned, resulting in thinner layers and longer aggregates.
机译：角蛋白是丝蛋白，但是我们在这项工作中，从羊毛水解水溶性角蛋白多肽可容易地吸附到水的表面，并因此可以用作表面活性生物材料的报告。中子反射测量用氘标记的帮助下被用于确定形成的多肽层的吸附量和分布。结果发现，界面层包括两个主要区域，18-25埃的致密的顶层和25-30埃的松散底层。顶部致密层的一半暴露在空气中与所述顶层的其余部分，浸渍在该水溶液中的扩散底层。两者的体积分数和层厚度增加与角蛋白溶液浓度为在约0.1g DM（-3）（2.1微米）那样将其视为高原略高于2毫克米（-2）的吸附量。增加[氯化钠]导致降低的表面吸附，伴随着顶层的变薄。低温TEM成像显示，角蛋白聚集体曾与半径范围从60埃到220埃的椭圆结构。椭球形深受SANS的支持，以140埃的大半径和60埃的小半径。随着[氯化钠]中，椭圆体变薄，但更长的时间，与来自表面吸附层所观察到的趋势一致的特征。这种不寻常的行为可以通过静电屏蔽效应来解释。随着盐浓度的增加，多肽链变得更硬和更易于对准，从而导致较薄的层和更长的聚集体。

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  • 来源
    《RSC Advances》 |2016年第107期|共10页
  • 作者

    Lu Zhiming; Pan Fang; Wang Dong; Campana Mario; Xu Hai; Tucker Ian M.; Petkov Jordan T.; Webster John; Lu Jian R.;

  • 作者单位

    Univ Manchester Sch Phys &

    Astron Biol Phys Lab Oxford Rd Manchester M13 9PL Lancs England;

    Univ Manchester Sch Phys &

    Astron Biol Phys Lab Oxford Rd Manchester M13 9PL Lancs England;

    China Univ Petr East China Ctr Bioengn &

    Biotechnol Qingdao 266580 Peoples R China;

    Univ Manchester Sch Phys &

    Astron Biol Phys Lab Oxford Rd Manchester M13 9PL Lancs England;

    China Univ Petr East China Ctr Bioengn &

    Biotechnol Qingdao 266580 Peoples R China;

    Unilever Res &

    Dev Lab Quarry Rd East Wirral CH63 2JW Merseyside England;

    Unilever Res &

    Dev Lab Quarry Rd East Wirral CH63 2JW Merseyside England;

    Rutherford Appleton Lab Sci &

    Technol Facil Council ISIS Neutron Facil Harwell Sci &

    Innovat Campus Didcot OX11 0QX Oxon England;

    Univ Manchester Sch Phys &

    Astron Biol Phys Lab Oxford Rd Manchester M13 9PL Lancs England;

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