Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system

Liu Sha; Zhang Hongyan; Liu Weimin; Zhou Bingjiang; Ma Qian; Ge Jiechao; Wu Jiasheng; Wang Pengfei

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Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system

机译：使用激光扫描共焦成像 - 表面等离子体谐振系统对生物细胞 - 小分子与金表面等离子体共振传感器的相互作用研究

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In our work, we investigated the interactions between a small molecule, folic acid, and biological cells through the interaction of folic acid and folate receptors using a laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) system. The changes of SPR peaks and cell concentration had good linear relationships, and fluorescence imaging provided further data. The detection limit was as low as 1.0 x 10(3) cells per mL, and linear coefficients were 0.95206, 0.95454, 0.94287, 0.98711, and 0.99228 for mouse lymphoma (L5178Y TK+/-) cells, mouse lymphoma (EL4) cells, mouse T lymphocytes (Cl.Ly 1+2-/9) cells, human lung cancer (A549) cells, and human oral epidermis carcinoma (KB) cells, respectively. The results indicated that the LSCI-SPR system has potential future application in analyzing small molecule-biological cell affinity and in acquiring quantitative parameters.

机译：在我们的作品中，我们通过使用激光扫描共焦成像 - 表面等离子体共振（LSCI-SPR）系统来研究小分子，叶酸和生物细胞之间的相互作用通过叶酸和叶酸受体的相互作用。 SPR峰和细胞浓度的变化具有良好的线性关系，荧光成像提供了进一步的数据。检出限量为每mL的1.0×10（3）个细胞，线性系数为0.95206,0.95454,0.94287,0.98711和0.99228，用于小鼠淋巴瘤（L5178Y TK +/-）细胞，小鼠淋巴瘤（EL4）细胞，小鼠 T淋巴细胞（Cl.ly1 + 2- / 9）细胞，人肺癌（A549）细胞和人口腔表皮癌（KB）细胞。结果表明，LSCI-SPR系统在分析小分子生物细胞亲和力和获取定量参数时具有潜在的未来应用。

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《RSC Advances》 |2016年第70期|共6页
作者
Liu Sha; Zhang Hongyan; Liu Weimin; Zhou Bingjiang; Ma Qian; Ge Jiechao; Wu Jiasheng; Wang Pengfei;
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作者单位

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

Chinese Acad Sci Tech Inst Phys &

Chem Key Lab Photochem Convers &

Optoelect Mat Beijing 100190 Peoples R China;

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正文语种 eng
中图分类化学;
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1. Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system [J] . Liu Sha, Zhang Hongyan, Liu Weimin, RSC Advances . 2016,第70期

机译：使用激光扫描共焦成像 - 表面等离子体谐振系统对生物细胞 - 小分子与金表面等离子体共振传感器的相互作用研究
2. Investigation of biological cell–small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system [J] . Sha Liu, Hongyan Zhang, Weimin Liu, RSC Advances . 2016,第70期

机译：使用激光扫描共焦成像 - 表面等离子体谐振系统对生物细胞 - 小分子与金表面等离子体共振传感器的相互作用研究
3. Investigation of biological cell-protein interactions using SPR sensor through laser scanning confocal imaging-surface plasmon resonance system [J] . Hongyan Zhang, Liquan Yang, Bingjiang Zhou, Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 2014,第Null期

机译：通过激光扫描共聚焦成像-表面等离振子共振系统使用SPR传感器研究生物细胞-蛋白质相互作用。
4. Ultrasensitive and selective detection of mercury (Ⅱ) in serum based on the gold film sensor using a laser scanning confocal imaging-surface plasmon resonance system in real time [C] . Sha Liu, Hongyan Zhang, Weimin Liu, Conference on Applied Optics and Photonics China . 2015

机译：基于金膜传感器的激光共聚焦成像-表面等离子体共振系统实时超灵敏和选择性地检测血清中的汞
5. Exploration of grating-based surface plasmon resonance systems for wave vector matching to enhance plasmon modes and preliminary surface plasmon-enhanced fluorescence interrogation. [D] . Johnson, Michael B. 2016

机译：探索基于光栅的表面等离子体激元共振系统，以进行波矢量匹配，以增强等离子体激元模式和初步的表面等离子体激元增强的荧光询问。
6. Surface Plasmon Resonance Imaging of Gold–Small Molecule Interactions Is Influenced by Refractive Index and Chemical Structures [O] . Zainab H Al Mubarak, Rajagopal Ramesh, Lin Liu, -1

机译：金-小分子相互作用的表面等离子体共振成像受折射率和化学结构的影响
7. Surface plasmon resonance imaging of gold–small molecule interactions is influenced by refractive index and chemical structures [O] . Zainab H. Al Mubarak, Rajagopal Ramesh, Lin Liu, 2015

机译：金小分子相互作用的表面等离子体共振成像受折射率和化学结构的影响
8. Novel Protein-Protein Interactions of the Yersinia pestis Type III secretion System Elucidated With a Matrix Analysis by Surface Plasmon Resonance and Mass Spectrometry [R] . Swietnicki, W. , O'Brien, S. , Holman, K. , 2004

机译：通过表面等离子体共振和质谱法进行基质分析阐明的鼠疫耶尔森氏菌III型分泌系统的新蛋白质 - 蛋白质相互作用

Investigation of biological cell-small molecule interactions with a gold surface plasmon resonance sensor using a laser scanning confocal imaging-surface plasmon resonance system

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