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首页> 外文期刊>RSC Advances >Specific interaction of platinated DNA and proteins by surface plasmon resonance imaging
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Specific interaction of platinated DNA and proteins by surface plasmon resonance imaging

机译：用表面等离子体共振成像用浸渍DNA和蛋白质的特异性相互作用

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摘要

The DNA-targeting platinum complex cisplatin is one of the most successful drugs for clinical treatment of solid tumors, and several new analogues of transplatin have shown cytostatic activity recently. The specific recognition of platinated DNA with cellular proteins is of great interest for better understanding of the pharmaceutical mechanisms. Herein, a surface plasmon resonance imaging (SPRi) method to differentiate the interaction between the protein human high mobility group box 1 (HMGB1) and DNAs, and human nuclear protein positive cofactor 4 (PC4) and DNAs has been developed. Four kinds of DNAs were immobilized on the gold films including platinated-DNA adducts (cisplatin and trans[PtCl2(NH3)(thiazole)] (trans-PtTz) damaged DNAs; referred to as cisPt-DNA1 and transPtTz-DNA2, respectively) and native DNAs (DNA1 and DNA2, as controls). The validation of the method has been proven by the specific recognition of HMGB1 and cisPt-DNA1 first. The results obtained indicated that the PC4 was more likely to bind to platinated DNAs (cisPt-DNA1 and transPtTz-DNA2) than the native DNAs. Temperature-dependent kinetics and thermodynamics revealed that the recognition behavior was not affected by a temperature changefrom 15 degrees C to 42 degrees C. This label-free method provides authentic results, as the controls can be simultaneously determined by a single chip under the same conditions, and this makes it suited for other high throughput analyses of interactions between drug-damaged DNAs and proteins to better understand the activity/inactivity mechanisms of drugs and drug screening/discovery.
机译：DNA靶向铂复合铂普拉丁是最成功的药物治疗实体瘤的临床治疗之一，最近几种新的转化素类似物表现出细胞抑制活性。具有细胞蛋白质的浸渍DNA的具体识别对于更好地理解药物机制非常有兴趣。这里，已经开发了一种分解蛋白质人高迁移率组盒1（HMGB1）和DNA的相互作用和人核蛋白质正辅助咖啡器4（PC4）和DNA的表面等离子体共振成像（SPRI）方法。将四种DNA固定在金膜上，包括氯丁DNA加合物（顺铂和反式[PTCL2（NH 3）（噻唑）]（Trans-PTTZ）受损DNA;分别称为Cispt-DNA1和Transpttz-DNA2）原生DNA（DNA1和DNA2，作为对照）。通过首先对HMGB1和CISPT-DNA1的特定识别证明了该方法的验证。得到的结果表明，PC4更可能与生物DNA粘合到浸渍的DNA（CISPT-DNA1和Transptttz-DNA2）。温度依赖性动力学和热力学显示，识别行为不受15摄氏度的温度变换的影响。该标签的方法提供了正宗的结果，因为控制器可以在相同条件下通过单个芯片同时确定。并且这使得其适用于药物受损的DNA和蛋白质之间的相互作用的其他高通量分析，以更好地了解药物和药物筛选/发现的活性/不活动机制。

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  • 来源
    《RSC Advances》 |2016年第26期|共7页
  • 作者

    Wang Xiao; Xu Jiying; Liu Chanjuan; Chen Yi;

  • 作者单位

    Chinese Acad Sci Inst Chem Key Lab Analyt Chem Living Biosyst Beijing 100190 Peoples R China;

    Chinese Acad Sci Inst Chem Key Lab Analyt Chem Living Biosyst Beijing 100190 Peoples R China;

    Chinese Acad Sci Inst Chem Key Lab Analyt Chem Living Biosyst Beijing 100190 Peoples R China;

    Chinese Acad Sci Inst Chem Key Lab Analyt Chem Living Biosyst Beijing 100190 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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