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Nano-topographic titanium modulates macrophage response in vitro and in an implant-associated rat infection model

机译：纳米地形钛在体外调节巨噬细胞反应和植入物相关的大鼠感染模型

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The macrophage-implant response plays a pivotal role in the interactions between implants and tissue involving inflammation and tissue remodeling. In this study, we investigated the proliferation, migration, and inflammatory cytokine secretion of macrophages adhering to titania nanotubes (TNT) and polished titanium (pTi) surfaces in a lipopolysaccharide (LPS)-simulated infection environment. An infection model in rats was used to analyze cell and bacterium responses to TNT and pTi implants in vivo. The in vitro results indicated that TNT surfaces restricted macrophage proliferation and migration and reduced proinflammatory cytokine secretion. Notably, LPS loading onto the TNT surface resulted in macrophage elongation with increased levels of secreted pro-inflammatory cytokines within 24 h followed by a decrease to the lowest level of all tested samples at 72 h. Analogously, increased amounts of inflammatory cytokines were observed for the TNT implants in vivo at 24 h with fewer detected at 72 h compared with pTi in the presence of Staphylococcus epidermidis (Se). Additionally, TNT implants exhibited lower total amounts of viable bacteria at 72 h than pTi implants. Furthermore, the tissues exhibited preferential spreading on TNT-Se implants at 72 h. These results suggested that the TNT surface in an infection environment could regulate the inflammatory response and promote tissue remodeling more effectively within the initial implantation compared with pTi. This study indicated the ability to construct functional nano-topographic surfaces by loading proteins or cytokines on implant surfaces that then could effectively modulate macrophages to support a healing process in lieu of chronic inflammation.
机译：巨噬细胞植入反应在植入物和组织之间的相互作用中起枢转作用，涉及炎症和组织重塑。在这项研究中，我们研究了粘附在脂多糖（LPS）刺激性的感染环境中粘附在二氧化钛纳米管（TNT）和抛光钛（PTI）表面的巨噬细胞的增殖，迁移和炎症细胞因子分泌。大鼠的感染模型用于分析细胞和细菌对TNT和PTI植入物的反应在体内。体外结果表明TNT表面限制巨噬细胞增殖和迁移和降低的促炎细胞因子分泌。值得注意的是，LPS加载到TNT表面上导致巨噬细胞伸长率随着24小时内的分泌型促炎细胞因子的水平增加，然后在72小时内降低到所有测试样品的最低水平。类似地，在葡萄球菌（SE）存在下，在72h比较PTI时，在24小时内观察到炎症细胞因子的增加量的炎性细胞因子。此外，TNT植入物在72小时的72小时下表现出较低的总量的活细菌。此外，组织在72小时的TNT-SE植入物上表现出优先蔓延。这些结果表明，与PTI相比，感染环境中的TNT表面可以调节炎症反应，并在初始植入中更有效地促进组织重塑。该研究表明，通过在植入表面上加载蛋白质或细胞因子来构建功能纳米地形表面的能力，然后可以有效地调节巨噬细胞以支持愈合过程代替慢性炎症。

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  • 来源
    《RSC Advances》 |2016年第113期|共9页
  • 作者

    Tan Jing; Zhao Chanjuan; Wang Yi; Li Yiting; Duan Ke; Wang Jianxin; Weng Jie; Feng Bo;

  • 作者单位

    Southwest Jiaotong Univ Sch Mat Sci &

    Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Univ Hosp 2 Chengdu Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Engn Key Lab Adv Technol Mat Minist Educ Chengdu Sichuan Peoples R China;

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