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Surface-enhanced Raman scattering based lateral flow immunochromatographic assay for sensitive influenza detection

机译：基于表面增强的拉曼散射横向流动免疫粒子学测定，用于敏感流感检测

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摘要

A sensitive surface-enhanced Raman scattering-based lateral flow immunochromatography test system (SERS-LFIA) was developed using multi-branched gold nanostars (AuNS) coated with a Raman active molecule, 4-aminothiophenol (ATP), as a signal reporter. The unique characteristic of the AuNS having multi-arms and surface roughness features allows high SERS performance in the system to be achieved via the increased bioconjugation sites and hot spot regions. To demonstrate the system performance, influenza A nucleoprotein was used as the target molecule. After a facile one-step synthesis, AuNS was tagged with the ATP molecule and conjugated to the antibody specific to influenza A nucleoprotein, and then used as both the SERS signal reporter and the detection probe in the system. It was demonstrated that the lower limit of detection using visual and SERS detection was 67 and 6.7 ng mL(-1), respectively. With the SERS detection signal, the detection sensitivity was significantly improved approximately 37 and 300 fold, as compared with fluorescence-based and conventional LFIA, respectively. The integrated SERS-LFIA platform reported here demonstrated an ultrahigh detection sensitivity, which could potentially facilitate a sensitive point-of-care test for diagnosis in many diseases.
机译：使用涂覆有拉曼活性分子，4-氨基噻吩醇（ATP）的多支化金纳盘（AUN），开发了一种基于表面增强的拉曼散射免疫层析试验系统（SERS-LFIA），作为信号报告。具有多臂和表面粗糙度特征的肛门的独特特征可以通过增加的生物杂交位点和热点区域实现高度的SERS性能。为了证明系统性能，使用流感核蛋白作为靶分子。在容易的一步合成之后，用ATP分子标记AUN，并与对甲核蛋白蛋白的特异性特异的抗体缀合，然后用作系统中的SERS信号报告器和检测探针。据证明，使用视觉和SERS检测的检测下限分别为67和6.7 ng mL（-1）。与SERS检测信号相比，检测灵敏度分别与荧光和常规LFIA相比，检测灵敏度显着提高约37和300倍。这里报道的集成SERS-LFIA平台展示了超高的检测灵敏度，这可能促进了许多疾病诊断的敏感点测试。

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  • 来源
    《RSC Advances》 |2016年第113期|共7页
  • 作者

    Maneeprakorn W.; Bamrungsap S.; Apiwat C.; Wiriyachaiporn N.;

  • 作者单位

    NSTDA Natl Nanotechnol Ctr NANOTEC Pathum Thani 12120 Thailand;

    NSTDA Natl Nanotechnol Ctr NANOTEC Pathum Thani 12120 Thailand;

    NSTDA Natl Nanotechnol Ctr NANOTEC Pathum Thani 12120 Thailand;

    NSTDA Natl Nanotechnol Ctr NANOTEC Pathum Thani 12120 Thailand;

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  • 正文语种 eng
  • 中图分类 化学;
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