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Plasmonic-ELISA: expanding horizons

机译：Plastonic-ELISA：扩张视野

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摘要

The enzyme-linked immunosorbent assay (ELISA) has been an integral part of in vitro diagnostic tests due to its high specificity, standard configuration, and convenient readout. The convergence of the plasmonic property, i.e. localized surface plasmon resonance (LSPR), of noble metal nanoparticles with the ELISA technique has established a novel category of immunoassay, known as "plasmonic ELISA" (pELISA). It has enabled the naked eye detection of various disease biomarkers down to attomolar concentration. The color development relies on the biocatalytic reaction mediated modulation of LSPR properties. Through this review we present various current state-of-art pELISA strategies adopted for naked-eye quantification of disease biomarkers along with their advantages, limitations and applicability. These strategies are broadly classified into following four categories based on the different means of LSPR modulation: (i) aggregation, (ii) controlled growth kinetics, (iii) metallization, and (iv) etching of metal nanoparticles. Furthermore, the paper highlights the different biocatalytic reactions and their role in plasmonic color development as a function of analyte concentration. The review ends with an elucidation of current challenges and future perspectives of pELISA precisely focusing on the need for development of next generation low cost point-of-care diagnostic kits.
机译：酶联免疫吸附试验（ELISA）由于其高特异性，标准配置和方便的读出而是体外诊断测试的一部分。具有ELISA技术的贵金属性质，即贵金属纳米颗粒的局部表面等离子体共振（LSPR）的收敛已经建立了一种新颖的免疫测定类别，称为“粒子激酶”（PELISA）。它使各种疾病生物标志物的肉眼检测能够降至抗摩托浓度。显色依赖于生物催化反应介导的LSPR性能调节。通过这篇综述，我们目前采用各种目前的艺术型佩萨策略，采用疾病生物标志物的肉眼定量，以及它们的优缺点，局限性和适用性。这些策略基于LSPR调制的不同手段大致分为以下四种类别：（i）聚集，（ii）控制的生长动力学，（III）金属纳米粒子的蚀刻蚀刻。此外，本文突出了不同的生物催化反应及其在等离子体显色作用中的作用作为分析物浓度的函数。审查结束，阐明了当前挑战的挑战和未来佩萨的观点精确地关注下一代低成本的护理诊断套件的发展。

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  • 来源
    《RSC Advances》 |2016年第88期|共17页
  • 作者

    Satija Jitendra; Punjabi Nirmal; Mishra Debasish; Mukherji Soumyo;

  • 作者单位

    VIT Univ Sch BioSci &

    Technol SBST Vellore 632014 Tamil Nadu India;

    Indian Inst Technol Dept Biosci &

    Bioengn Bombay 400076 Maharashtra India;

    VIT Univ Sch BioSci &

    Technol SBST Vellore 632014 Tamil Nadu India;

    Indian Inst Technol Dept Biosci &

    Bioengn Bombay 400076 Maharashtra India;

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  • 正文语种 eng
  • 中图分类 化学;
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