• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Quantum dots-based fluoroimmunoassay for the simultaneous detection of clothianidin and thiacloprid in environmental and agricultural samples
【24h】

Quantum dots-based fluoroimmunoassay for the simultaneous detection of clothianidin and thiacloprid in environmental and agricultural samples

机译：基于量子点的含氟免疫测定，用于同时检测衣物和农业样品中的衣物和噻菌药

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Quantum dots (QDs), luminescent semiconductor nanocrystals, have the potential to simplify the performance of multi-analyte analysis. In this work, a novel indirect competitive fluorescence-linked immunosorbent assay (FLISA) based on QDs for the detection of clothianidin and thiacloprid simultaneously in environmental and agricultural samples were performed in a single well of the microtiter plate. The QDs-labeled antibody conjugates, which consists of CdSe/ZnS core-shell QDs and polyclonal antibodies, were prepared through the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) coupling method. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of the FLISA were 12.5 and 0.3 ng mL(-1) for clothianidin, and 9.27 and 0.4 ng mL(-1) for thiacloprid, respectively. The cross-reactivities of the FLISA with the analogues of clothianidin and thiacloprid were negligible except for dinotefuran (10.4% for clothianidin). The spiked recoveries were 73.3% to 113.1% with relative standard deviations (RSDs) of 2.7 to 13.3% for the detection of clothianidin and thiacloprid in river water, soil, cabbage, rice and tomato. Furthermore, the results of FLISA for the authentic samples correlated well with those obtained by HPLC. The proposed FLISA is a satisfactory tool for rapid, sensitive, and quantitative detection of two pesticides simultaneously in agricultural and environmental samples. This study provides a strategy for the development of QDs-based fluoroimmunoassay for the quantitative analysis of two pesticides.
机译：量子点（QDS），发光半导体纳米晶体具有潜力，以简化多分析物分析的性能。在这项工作中，在微量滴定板的单个孔中进行基于在环境和农业样品中检测的QDS的新型间接竞争荧光链接免疫吸附测定（FLISA）。由CDSE / ZnS核 - 壳QDS和多克隆抗体组成的QDS标记的抗体缀合物通过1-乙基-3-（3-二甲基氨基丙基）碳二亚胺酰胺（EDC）偶联方法制备。在最佳条件下，FLISA的半最大抑制浓度（IC50）和烟火的检测（LOD，IC10）为3.5和0.3ng（-1），以及9.27和0.4 ng ml（-1）分别为噻虫草。除了Dinotefuran外，FLISA与噻虫蛋白和噻虫草的类似物的交叉反射性可以忽略不计（对于薄薄糖蛋白）。尖刺的回收率为73.3％至113.1％，相对标准偏差（RSD）为河水，土壤，卷心菜，稻米和番茄中的披肩和噻虫草的相对标准偏差（RSD）为2.7至13.3％。此外，对于通过HPLC获得的那些，Flisa的Flisa结果良好良好。该拟议的FLISA是一种令人满意的工具，用于在农业和环境样品中同时快速，敏感和定量检测两种农药。本研究为开发基于QDS的FluoroImmunoassay进行了策略，用于两种杀虫剂的定量分析。

著录项

  • 来源
    《RSC Advances》 |2015年第4期|共6页
  • 作者

    Li Ming; Ma Ming; Hua Xiude; Shi Haiyan; Wang Qiuxia; Wang Minghua;

  • 作者单位

    Nanjing Agr Univ Coll Plant Protect Dept Pesticide Sci State &

    Local Joint Engn Res C Minist Educ Key Lab Integrated Management Crop Di Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Plant Protect Dept Pesticide Sci State &

    Local Joint Engn Res C Minist Educ Key Lab Integrated Management Crop Di Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Plant Protect Dept Pesticide Sci State &

    Local Joint Engn Res C Minist Educ Key Lab Integrated Management Crop Di Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Plant Protect Dept Pesticide Sci State &

    Local Joint Engn Res C Minist Educ Key Lab Integrated Management Crop Di Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Plant Protect Dept Pesticide Sci State &

    Local Joint Engn Res C Minist Educ Key Lab Integrated Management Crop Di Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Plant Protect Dept Pesticide Sci State &

    Local Joint Engn Res C Minist Educ Key Lab Integrated Management Crop Di Nanjing 210095 Jiangsu Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号