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首页> 外文期刊>RSC Advances >Crucial role of intestinal barrier in the formation of transgenerational toxicity in quantum dot exposed nematodes Caenorhabditis elegans
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Crucial role of intestinal barrier in the formation of transgenerational toxicity in quantum dot exposed nematodes Caenorhabditis elegans

机译：肠道屏障在量子点曝光的转基因毒性形成中的关键作用暴露的NematodeCaenorhabditis elegans

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摘要

Previous studies have demonstrated that long-term exposure to quantum dots (QDs) causes adverse effects on organisms. However, the possible transgenerational toxicity of QDs and the underlying mechanisms are still largely unclear. In the present study, we employed Caenorhabditis elegans as an in vivo toxicity assessment assay system. With the aid of locomotion behavior and intestinal reactive oxygen species (ROS) production as endpoints, we found that exposure to CdTe QDs (2.5-20 mg L~(-1) ) caused the formation of transgenerational toxicity in nematodes. In nematodes with the transgenerational toxicity of CdTe QDs, we observed that, besides the pharynx and intestine, CdTe QDs could also be accumulated in reproductive organs such as the gonad and embryos of the exposed animals. Moreover, ZnS coating could reduce the toxicity of CdTe QDs by maintaining the normal function of the intestinal barrier, suggesting the crucial role of the intestinal barrier against transgenerational toxicity of CdTe QDs. For the molecular mechanisms of the observed transgenerational toxicity of CdTe QDs, our results suggest that clk-1, isp-1, and daf-2 might positively regulate the formation of transgenerational toxicity in CdTe QD exposed nematodes. Mutation or intestine-specific RNAi of clk-1, isp-1,or daf-2 genes caused the formation of a resistant property of the nematodes to transgenerational toxicity of CdTe QDs, and prevented the translocation of CdTe QDs from the exposed nematodes to their progeny. Our data will provide insight to the transgenerational behavior of engineered nanomaterials and the possible underlying mechanisms in organisms.
机译：以前的研究表明，长期暴露于量子点（QDS）对生物体产生不利影响。然而，QDS的可能的转基因毒性和潜在机制仍然很大程度上不清楚。在本研究中，我们在体内毒性评估测定系统中使用Caenorhabditis elegans。借助当机车行为和肠道活性氧（ROS）生产作为终点，我们发现暴露于CDTE QD（2.5-20mg L〜（-1））导致线虫中转基因毒性的形成。在随着CDTE QD的转基因毒性的线虫中，我们观察到，除了咽和肠之外，CDTE QD也可以累积在繁殖器官中，例如暴露动物的GONAD和胚胎。此外，通过维持肠道屏障的正常功能，ZnS涂层可以降低CdTe QD的毒性，表明肠道屏障对CDTE QDS的转基因毒性的关键作用。对于CDTE QD的观察到的转基因毒性的分子机制，我们的结果表明CLK-1，ISP-1和DAF-2可能会积极调节CDTE QD暴露的线虫中转基因毒性的形成。 CLK-1，ISP-1或DAF-2基因的突变或肠特异性RNAi导致线虫的抗性特性形成CDTE QD的转基因毒性，并阻止了从暴露的线虫的CDTE QD转移到其上后代。我们的数据将提供对工程纳米材料的转基因行为以及有机体中可能的潜在机制的洞察力。

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  • 来源
    《RSC Advances》 |2015年第114期|共10页
  • 作者

    Zhifei Liu; Xuefeng Zhou; Qiuli Wu;

  • 作者单位

    Key Laboratory of Environmental Medicine Engineering in Ministry of Education Medical School Southeast University Nanjing 210009 China.;

    Key Laboratory of Environmental Medicine Engineering in Ministry of Education Medical School Southeast University Nanjing 210009 China.;

    Key Laboratory of Environmental Medicine Engineering in Ministry of Education Medical School Southeast University Nanjing 210009 China.;

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  • 正文语种 eng
  • 中图分类 化学;
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