• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Analyzing the effects of protecting osmolytes on solute-water interactions by solvatochromic comparison method: II. Globular proteins
【24h】

Analyzing the effects of protecting osmolytes on solute-water interactions by solvatochromic comparison method: II. Globular proteins

机译：溶胶多变色比较法分析保护渗透渗透渗水相互作用的影响：II。 球状蛋白

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Partitioning of 11 globular proteins was examined in aqueous dextran-PEG-sodium/potassium phosphate buffer (0.01 M K/NaPB, pH 7.4) two-phase systems (ATPSs) containing 0.5 M sorbitol. The data obtained were analyzed together with those reported previously for the same proteins in osmolyte-free ATPS and ATPS containing 0.5 M sucrose, TMAO, or trehalose. It was found that all the partition coefficients for proteins determined in the presence of 0.5 M of different osmolytes and in the absence of osmolytes may be described in terms of the differences between solvent properties of the coexisting phases. Solute-specific coefficients characterizing different types of solute-solvent interactions were calculated for each protein. These solute-specific coefficients are linearly interrelated implying cooperativity of different types of protein-water interactions. The data obtained indicate the lack of any association of the aforementioned osmolytes at concentration of 0.5 M with proteins. Computational analysis of one of the solute-specific coefficient Ss-values characterizing dipole-dipole protein-water interactions shows that it is determined by the peculiarities of protein surface.
机译：11种球状蛋白的划分在水性葡聚糖-PEG-磷酸钠/磷酸钾缓冲液（0.01M K / NAPB，pH 7.4）中含有0.5M山梨糖醇的两相系统（ATPSs）进行了研究。获得的分析与这些在一起的数据预先在无渗压剂-ATPS含有0.5M蔗糖，TMAO，或海藻糖相同蛋白质和ATPS报道。结果发现，所有在不同的渗压剂的0.5M的存在下和在不存在渗透物确定蛋白的分配系数可以以共存相的溶剂性质之间的差异进行描述。表征不同类型的溶质 - 溶剂相互作用的溶质特异性系数计算出各蛋白。这些特定的溶质系数线性相互关联意味着不同类型的蛋白与水的相互作用的协同性。获得的数据表明，缺乏在与蛋白质的0.5M的浓度在上述渗透剂的任何关联的。表征偶极 - 偶极蛋白与水的相互作用表明，它是由蛋白质表面的特殊性确定的特定溶质系数SS-值中的一个的计算分析。

著录项

  • 来源
    《RSC Advances》 |2015年第73期|共12页
  • 作者

    Ferreira Luisa A.; Fan Xiao; Madeira Pedro P.; Kurgan Lukasz; Uversky Vladimir N.; Zaslavsky Boris Y.;

  • 作者单位

    Analiza Inc Cleveland OH 44114 USA;

    Univ Alberta Dept Elect &

    Comp Engn Edmonton AB T6G 2M7 Canada;

    Univ Porto Fac Engn Dept Chem Engn Lab Separat &

    React Engn P-4200465 Oporto Portugal;

    Univ Alberta Dept Elect &

    Comp Engn Edmonton AB T6G 2M7 Canada;

    Univ S Florida Morsani Coll Med Dept Mol Med Tampa FL 33612 USA;

    Analiza Inc Cleveland OH 44114 USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号