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首页> 外文期刊>RSC Advances >Simple and rapid preparation of orange-yellow fluorescent gold nanoclusters using DL-homocysteine as a reducing/stabilizing reagent and their application in cancer cell imaging
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Simple and rapid preparation of orange-yellow fluorescent gold nanoclusters using DL-homocysteine as a reducing/stabilizing reagent and their application in cancer cell imaging

机译：使用DL-Homocysteine作为还原/稳定试剂的简单快速制备橙黄荧光金纳米蛋白及其在癌细胞成像中的应用

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摘要

A simple, convenient and fast preparation for successful fabrication of water-soluble DL-homocysteine capped Au nanoclusters (hcy-AuNCs) was demonstrated. This preparation allowed the generation of water-soluble Au NCs within a short time of 15 min. The resulting hcy-AuNCs were characterized by photoluminescence, UV-Vis absorption, X-ray photoelectron spectroscopy, and transmission electron microscopy (TEM). The mean diameter of hcy-AuNCs was found to be 1.6 +/- 0.2 nm. The Au NCs exhibited an orange-yellow fluorescence emission at 560 nm with a large Stokes shift of 120 nm, a quantum yield of 3.01% and a good stability over the physiologically relevant pH range and ionic strength. Furthermore, cytotoxicity studies showed that the Au NCs exhibited negligible effects in altering cell proliferation or triggering apoptosis. Cancer cell imaging of HeLa cell lines indicated that the obtained Au NCs could serve as a promising fluorescent bioprobe for bioimaging. This strategy, based on the one-step preparation using DL-homocysteine as a reducing/stabilizing reagent, may offer a novel approach to fabricate other water-soluble metal nanoclusters for application in biolabelling and bioimaging.
机译：对成功制备水溶性DL-同型内膜封端的Au纳米能器（Hcy-auncs）的简单，方便和快速制备。该制剂允许在15分钟的短时间内产生水溶性AU NC。通过光致发光，UV-Vis吸收，X射线光电子体光谱和透射电子显微镜（TEM）表征得到的HCY-AUND。发现Hcy-auncs的平均直径为1.6 +/- 0.2nm。 Au NCS在560nm处表现出橙黄色荧光发射，大小速度偏移120nm，量子产率为3.01％，对生理相关的pH范围和离子强度稳定性良好。此外，细胞毒性研究表明，AU NCs在改变细胞增殖或触发细胞凋亡方面表现出可忽略的影响。 HeLa细胞系的癌细胞成像表明，所获得的Au NC可以用作BioMimaging的有前途的荧光生物探针。基于使用D1-同型半胱氨酸作为还原/稳定试剂的单步制剂的该策略可以提供一种用于制造其他水溶性金属纳米能器的新方法，用于在生物标记和生物体中应用。

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  • 来源
    《RSC Advances》 |2015年第15期|共6页
  • 作者

    Xu Shouming; Yang Hong; Zhao Kang; Li Jianguo; Mei Liyun; Xie Yun; Deng Anping;

  • 作者单位

    Soochow Univ Coll Chem Chem Engn &

    Mat Sci Key Lab Hlth Chem &

    Mol Diag Suzhou Suzhou 215123 Peoples R China;

    Soochow Univ Coll Pharm Sci Suzhou 215123 Peoples R China;

    Soochow Univ Coll Chem Chem Engn &

    Mat Sci Key Lab Hlth Chem &

    Mol Diag Suzhou Suzhou 215123 Peoples R China;

    Soochow Univ Coll Chem Chem Engn &

    Mat Sci Key Lab Hlth Chem &

    Mol Diag Suzhou Suzhou 215123 Peoples R China;

    Soochow Univ Coll Chem Chem Engn &

    Mat Sci Key Lab Hlth Chem &

    Mol Diag Suzhou Suzhou 215123 Peoples R China;

    Soochow Univ Coll Chem Chem Engn &

    Mat Sci Key Lab Hlth Chem &

    Mol Diag Suzhou Suzhou 215123 Peoples R China;

    Soochow Univ Coll Chem Chem Engn &

    Mat Sci Key Lab Hlth Chem &

    Mol Diag Suzhou Suzhou 215123 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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