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Deep eutectic solvents as a new class of draw agent to enrich low abundance DNA and proteins using forward osmosis

机译:深红色的溶剂作为一种新的吸引剂,以丰富低丰度DNA和蛋白质使用前渗透剂

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摘要

The present study explores the potential of green and sustainable deep eutectic solvents (DESs) as an alternative energy source for concentrating proteins (>6 times) and DNA (>3 times) using forward osmosis (FO). The distinct colligative properties like high osmotic pressure (P >300 atm) and low water activity (aw, #0.1) of the DESs make them an ideal draw solution in the FO process. These novel draw solutions in combination with a composite semi-permeable membrane were successfully tested for enriching biomacromolecules. Recovery of the draw agent was achieved by chilling the diluted solution after FO, which could then be reused in the process.
机译:本研究探讨了绿色和可持续深度共晶溶剂(DESS)作为替代渗透蛋白(> 6次)和DNA(> 3次)的替代能源的潜力,使用前渗透(FO)。 DES的高渗透压(P> 300atm)和低水力活动(AW,#0.1)等不同的戏剧性质使其成为FO过程中的理想绘制溶液。 这些新颖的绘制溶液与复合半透膜组合的用于富集生物致摩洛。 通过冷却稀释的溶液在佛化之后降低稀释的溶液,可以在该方法中重复使用的溶液来实现。

著录项

  • 来源
    《RSC Advances》 |2015年第109期|共6页
  • 作者单位

    Marine Biotechnology &

    Ecology Division CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) G. B. Marg Bhavnagar 364 002 India.;

    Reverse Osmosis Membrane Division CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) India;

    Reverse Osmosis Membrane Division CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI) India;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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