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Improving alkaline pretreatment method for preparation of whole rice waste biomass feedstock and bioethanol production

机译：改善碱性预处理方法制备全水稻废物原料和生物乙醇生产

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摘要

Efficient conversion of fermentable sugars from cheap lignocellulosic biomass is currently needed for viable ethanol production technology. This study investigates the efficiency of individual and multiple chemical pretreatment for the saccharification of whole rice waste biomass (RWB). Individual alkaline pretreatment combined with autoclaving provided a maximum yield of 505 mg of reducing sugar with 69.2% hydrolysis yield after two- step enzymatic hydrolysis using 20 filter paperase (FPU) g(-1) of RWB. Sequential pretreatment of acidified sodium chlorite and sodium bicarbonate to alkaline pretreated RWB resulted in maximum yield of 725 mg of reducing sugar with hydrolysis yield of 90.6% using a lower enzyme dose (10 FPU g(-1) of RWB), indicating the feasibility of the process. The effects of different pretreatments on chemical composition and structural complexity of whole RWB and its relation with saccharification were investigated using various analytical techniques. Finally, the enzymatic hydrolysates of pretreated RWB were found suitable for ethanol production. The maximum sugar consumption and ethanol yield by Saccharomyces cerevisiae SR8 were 95% and 0.465 g g(-1) of sugar, respectively. The developed sequential pretreatment showed significant delignification and saccharification of whole RWB at low enzyme dosage. Also efficient conversion of the hydrolysate to ethanol without detoxification demonstrated the feasibility of the process.
机译：有效转化可发酵糖的可发酵糖，目前需要可行的乙醇生产技术。本研究研究了全水稻废物生物量（RWB）糖化的个体和多种化学预处理的效率。单个碱性预处理与高压灭菌合并提供505mg还原糖的最大收率，其使用20次滤成纸酶（FPU）G（-1）的RWB酶水解后的69.2％的水解产率。顺序预处理酸化亚氯酸钠和碳酸氢钠与碱预处理的RWB导致最大收率为725mg的还原糖，水解产率为90.6％，使用较低酶剂量（10fpu g（-1）RWB），表明可行性这个过程。采用各种分析技术研究了不同预处理对整个RWB化学成分和结构复杂性的影响及其与糖化的关系。最后，发现预处理RWB的酶水解产物适用于乙醇生产。 Saccharomyces Cerevisiae SR8的最大糖消耗和乙醇产量分别为95％和0.465g g（-1）糖。发育的顺序预处理显示出在低酶剂量下整个RWB的显着脱果和糖化。在不解毒的情况下，还有效地将水解产物转化为乙醇，证明了该方法的可行性。

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  • 来源
    《RSC Advances》 |2015年第118期|共9页
  • 作者

    Saratale Ganesh D.; Oh Min-Kyu;

  • 作者单位

    Korea Univ Dept Chem &

    Biol Engn Seoul 136713 South Korea;

    Korea Univ Dept Chem &

    Biol Engn Seoul 136713 South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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