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首页> 外文期刊>RSC Advances >Direct determination of astragalosides and isoflavonoids from fresh Astragalus membranaceus hairy root cultures by high speed homogenization coupled with cavitation-accelerated extraction followed by liquid chromatography-tandem mass spectrometry
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Direct determination of astragalosides and isoflavonoids from fresh Astragalus membranaceus hairy root cultures by high speed homogenization coupled with cavitation-accelerated extraction followed by liquid chromatography-tandem mass spectrometry

机译：通过高速均化与液相色谱 - 串联质谱，通过高速均化直接测定来自新鲜黄芪膜毛发根培养物的黄芪和异黄酮。液相色谱 - 串联质谱法

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摘要

A direct analysis approach for plant in vitro cultures, namely, high speed homogenization coupled with cavitation-accelerated extraction (HSH-CAE) followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), was developed for the simultaneous determination of six astragalosides and five isoflavonoids in Astragalus membranaceus hairy root cultures (AMHRCs). In comparison to reported Soxhlet extraction (SE) and ultrasound-assisted extraction (UAE) methods, the proposed sample preparation procedure (HSH-CAE) offers significant improvements with regard to simplicity in operation (elimination of biomass drying and grinding), high efficiency, enhanced yield and green aspects in terms of saving energy cost and minimizing the generation of waste. In addition, the HSH-CAE mechanism was clarified via cytohistological studies of samples at cellular/tissular levels. Moreover, the established LC-MS/MS method provided linearity with correlation coefficients above 0.9991, limit of detections (LODs) below 1.77 ng mL(-1), relative standard deviations (RSDs) below 6.01%, and recoveries above 96.84%. Furthermore, the proposed HSH-CAE-LC-MS/MS method was also successfully applied for screening high-productive AMHRCs. Overall, this study opened up a new avenue for the direct determination of secondary metabolic profiles from fresh plant in vitro cultures, which was valuable for improving the quality control of plant cell/organ cultures and shed light on the metabolomics analysis from biological samples.
机译：植物体外培养的直接分析方法，即高速均质，与空化加速萃取（HSH-CAE），然后进行液相色谱 - 串联质谱（LC-MS / MS），同时测定六个黄芪和黄芪和五种异黄酮在黄芪膜毛发根培养物（AMHRC）。与报道的SOXHLET提取（SE）和超声辅助提取（UAE）方法相比，所提出的样品制备程序（HSH-CAE）在操作方面的简单性（消除生物质干燥和研磨）方面提供了显着的改进，高效，在节省能源成本和最小化废物的产生方面提高产量和绿色方面。此外，通过细胞/组织水平的样品的细胞学研究阐明了HSH-CAE机制。此外，所建立的LC-MS / MS方法提供了具有高于0.9991的相关系数的线性度，检测极限（LOD）低于1.77 Ng mL（-1），相对标准差（RSD）低于6.01％，回收率高于96.84％。此外，还成功地应用了所提出的HSH-CAE-LC-MS / MS方法以筛选高生产率的AMHRC。总体而言，这项研究开辟了新的途径，用于直接测定新的植物中的次级代谢谱的体外培养物，这对于改善植物细胞/器官培养物的质量控制和脱光对生物样品的代谢组科分析有价值。

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  • 来源
    《RSC Advances》 |2015年第44期|共10页
  • 作者

    Jiao Jiao; Gai Qing-Yan; Luo Meng; Peng Xiao; Zhao Chun-Jian; Fu Yu-Jie; Ma Wei;

  • 作者单位

    Northeast Forestry Univ State Key Lab Tree Genet &

    Breeding Harbin 150040 Peoples R China;

    Northeast Forestry Univ Minist Educ Key Lab Forest Plant Ecol Harbin 150040 Peoples R China;

    Northeast Forestry Univ Minist Educ Key Lab Forest Plant Ecol Harbin 150040 Peoples R China;

    Northeast Forestry Univ Minist Educ Key Lab Forest Plant Ecol Harbin 150040 Peoples R China;

    Northeast Forestry Univ Minist Educ Key Lab Forest Plant Ecol Harbin 150040 Peoples R China;

    Northeast Forestry Univ Minist Educ Key Lab Forest Plant Ecol Harbin 150040 Peoples R China;

    Northeast Forestry Univ State Key Lab Tree Genet &

    Breeding Harbin 150040 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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