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Real-time selective monitoring of allergenic Aspergillus molds using pentameric antibody-immobilized single-walled carbon nanotube-field effect transistors

机译：使用五聚体抗体固定的单壁碳纳米管 - 场效应晶体管的过敏性曲霉菌模具的实时选择性监测

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摘要

Airborne fungus, including Aspergillus species, is one of the major causes of human asthma. Conventional immunoassay or DNA-sequencing techniques, although widely used, are usually labor-intensive, time-consuming and expensive. In this paper, we demonstrate a sensor for the rapid detection of Aspergillus niger, a well-known allergenic fungal species, using single-walled carbon nanotube (SWNT) field effect transistors (FETs) functionalized with pentameric antibodies that specifically bind to Aspergillus species. This strategy resulted in the real time and highly sensitive and selective detection of Aspergillus due to an electrostatic gating effect from the Aspergillus fungus. This mechanism is in contrast to a previously reported Aspergillus sensor, which was based on mobility modulation from Aspergillus adsorption. Also, our sensor shows a much wider detection range from 0.5 pg mL(-1) to 10 mg mL(-1) with a lower detection limit of 0.3 pg mL(-1). The resulting SWNT-FET was able to selectively detect Aspergillus molds in the presence of more concentrated amounts of other mold species such as Alternaria alternata, Cladosporium cladosporioides, and Penicillium chrysogenum. We expect that our results can be used in real-time monitoring of the indoor air quality of a variety of public facilities for the elderly and children, who are more vulnerable to environmental biohazards.
机译：空中真菌，包括曲霉属物种，是人类哮喘的主要原因之一。常规免疫测定或DNA测序技术虽然广泛使用，通常是劳动密集型，耗时且昂贵的。在本文中，我们证明了一种用于快速检测Aspergillus Niger，一种众所周知的过敏性真菌物种的传感器，所述单壁碳纳米管（SWNT）场效应晶体管（FET）与特异性结合Aspergillus物种的五聚体抗体官能化。由于曲霉属真菌的静电门控效果，该策略导致了对曲霉的实时和高度敏感和选择性检测。该机制与先前报告的曲霉菌传感器相反，基于曲霉吸附的迁移率调节。此外，我们的传感器显示出从0.5pg ml（-1）至10mg ml（-1）的更宽的检测范围，检测限为0.3pg ml（-1）。所得的SWNT-FET能够在更浓度的其他模具物种（如alterararia alterna，cladosporium cladosophers和Penicillium chrysogenum）存在中选择性地检测曲霉菌模具。我们预计我们的结果可用于对老年人和儿童各种公共设施的室内空气质量的实时监测，他们更容易受到环境生物危害的群体。

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  • 来源
    《RSC Advances》 |2015年第20期|共8页
  • 作者

    Jin Joon-Hyung; Kim Junhyup; Jeon Taejin; Shin Su-Kyoung; Sohn Jong-Ryeul; Yi Hana; Lee Byung Yang;

  • 作者单位

    Korea Univ Dept Mech Engn Seoul 136713 South Korea;

    Korea Univ Dept Mech Engn Seoul 136713 South Korea;

    Korea Univ Dept Mech Engn Seoul 136713 South Korea;

    Korea Univ Grad Sch Dept Publ Hlth Sci Seoul 136703 South Korea;

    Korea Univ Dept Environm Hlth Seoul 136703 South Korea;

    Korea Univ Grad Sch Dept Publ Hlth Sci Seoul 136703 South Korea;

    Korea Univ Dept Mech Engn Seoul 136713 South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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