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MCR-ALS as an effective tool for monitoring subsequent phase transitions in pure and doped DPPC liposomes

机译：MCR-AS作为用于监测纯和掺杂DPPC脂质体中随后的相变的有效工具

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摘要

The MCR-ALS method was applied to increase the structural information derived from infrared spectra of pure and doped dipalmitoylphosphatidylcholine (DPPC) liposomes. Pure DPPC vesicles and mixed ones with long-chain homologues of the phenol substituted by a tert-butyl moiety were investigated using the attenuated total reflectance Fourier-transfer infrared spectroscopy (FTIR-ATR). A combination of pure spectral profiles with pure concentration ones, which were derived from MCR-ALS calculations, enabled us to discuss in detail the structural characteristics of each pure phase state, which occur during a heating of pure as well as mixed lipid systems. Additionally, alterations of relative concentrations of subsequent components, which were represented by different pure phase states, associated with individual phase transitions, were determined in both pure and mixed DPPC systems. As far as we known, it is the first application of the MCR-ALS calculation in analysis of FTIR-ATR spectra of DPPC membranes.
机译：施用MCR-ALS方法以增加衍生自纯和掺杂的二硫代酰基磷脂酰胆碱（DPPC）脂质体的红外光谱的结构信息。使用衰减的总反射率傅里叶转移红外光谱（FTIR-ATR）研究纯DPPC囊泡和具有由叔丁基部分取代的苯酚的长链同源物的混合系。具有纯浓度的纯谱分析的组合，其衍生自MCR-Als计算，使我们能够详细讨论每个纯相位状态的结构特征，这在加热纯的和混合脂质系统期间发生。另外，在纯和混合的DPPC系统中测定与单个相转变相关的不同纯相态表示的后续组分的相对浓度的改变。据我们所知，它是DPPC膜FTIR-ATR光谱分析中的MCR-ALS计算的第一次应用。

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