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首页> 外文期刊>RSC Advances >Deuterated hydrazino-s-triazine as highly-efficient labelling reagent for glycan relative quantification analysis using electrospray ionization mass spectrometry
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Deuterated hydrazino-s-triazine as highly-efficient labelling reagent for glycan relative quantification analysis using electrospray ionization mass spectrometry

机译：氘代Hydrazina-Tri嗪作为使用电喷雾电离质谱法的聚糖相对定量分析的高效标记试剂

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摘要

Herein, an innovative stable-isotope relative quantification strategy for N-glycans was achieved using a self-designed non-reductive hydrazino-s-triazine deuterated derivative as a labelling reagent combined with mass spectrometry. As much as a 20 Da mass shift could effectively distinguish different forms of N-glycans labelled with normal and heavy hydrazino-s-triazine at the reducing end. Especially for glycans with high molecular weight, qualitative identification could be significantly simplified on account of avoiding isotopic distributions overlapping in the mass spectra. Meanwhile, a deuterium derivative with high purity guaranteed the accuracy of the relative quantification. The obviously undifferentiated response toward normal and heavy hydrazino-s-triazine labelled glycans in electrospray ionization mass spectrometry confirmed the feasibility and reliability of our proposed strategy, which was further demonstrated by relative quantification of the mixtures of labelled N-glycans cleaved from ovalbumin as a model sample. Finally, we adopted human serum as a complex sample and successfully achieved highly accurate detection of 48 N-glycans. The deuterated hydrazino-s-triazine labelling reagent exhibited a great potential to promote the development of highly-efficient, accurate and reliable N-glycan relative quantification in pharmacy and diagnosis research.
机译：在此，使用自行设计的非还原肼基-s-三嗪作为氘化的标记试剂与质谱联用衍生物达到对N-聚糖的创新稳定同位素相对定量的策略。多达20沓质量偏移可以有效区分不同的形式标记有在还原端正常和重肼基-s-三嗪的N-聚糖。特别是对于具有高分子量的聚糖，定性鉴定可以显著简化对帐户避免同位素分布在质谱重叠。同时，高纯度的氘衍生物保证相对定量的精度。在电喷雾离子化质谱法对正常和重肼基-s-三嗪标记的聚糖的明显未分化的响应证实了我们提出的策略，其进一步通过标记的N-聚糖的从卵清蛋白裂解作为混合物的相对定量证明的可行性和可靠性模型样品。最后，我们通过人血清作为样品的复杂和成功实现的48 N-聚糖高度精确的检测。氘代肼基-s-三嗪标记试剂表现出很大的潜力，以促进高效，准确，可靠的N-聚糖相对定量的发展在药学和诊断研究。

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  • 来源
    《RSC Advances》 |2015年第97期|共6页
  • 作者

    Zhao Ming-Zhe; Tie Cai; Zhang Yi-Wei; Deng Yan; Zhang Fang-Ting; Zhou Ying-Lin; Zhang Xin-Xiang;

  • 作者单位

    Peking Univ Coll Chem Inst Analyt Chem Key Lab Biochem &

    Mol Engn Minist Educ BNLMS Beijing 100871 Peoples R China;

    Peking Union Med Coll Inst Mat Med State Key Lab Bioact Subst &

    Funct Nat Med Beijing 100050 Peoples R China;

    Peking Univ Coll Chem Inst Analyt Chem Key Lab Biochem &

    Mol Engn Minist Educ BNLMS Beijing 100871 Peoples R China;

    Peking Univ Coll Chem Inst Analyt Chem Key Lab Biochem &

    Mol Engn Minist Educ BNLMS Beijing 100871 Peoples R China;

    Peking Univ Coll Chem Inst Analyt Chem Key Lab Biochem &

    Mol Engn Minist Educ BNLMS Beijing 100871 Peoples R China;

    Peking Univ Coll Chem Inst Analyt Chem Key Lab Biochem &

    Mol Engn Minist Educ BNLMS Beijing 100871 Peoples R China;

    Peking Univ Coll Chem Inst Analyt Chem Key Lab Biochem &

    Mol Engn Minist Educ BNLMS Beijing 100871 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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