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Catanionic vesicles and DNA complexes: a strategy towards novel gene delivery systems

机译：Catanionic囊泡和DNA复合物：新型基因递送系统的策略

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摘要

Catanionic vesicles are appealing vectors in non-viral gene transfection. They possess high kinetic stability and the preparation procedures are easy and cheap. In addition, their size and charge are easily modulated by varying the mole ratio between the components. For these reasons, we investigated the interactions between positively charged catanionic vesicles made of didecyldimethylammonium bromide (DiDAB) and 8-hexadecyl sulfate (8-SHS) with calf thymus DNA. Strongly associating complexes are obtained and their structure depends on DNA content. At low concentration, DNA/vesicles complexes are stable, with features very similar to bare vesicles. In the presence of DNA, multi-lamellar entities are formed; the process is promoted by the aggregation and rearrangement of DNA/vesicle complexes. Surface adsorption onto vesicles increases in proportion to DNA content. In such conditions, zeta-potential abruptly decreases, because of the formation of large clusters in which the vesicular identity is retained. Thereafter, precipitation occurs. The solid obtained accordingly is a lamellar phase with DNA sandwiched between the lamellae. The D-1 distance between DNA molecules in the lamellar phase and the precipitate composition depend on the biopolymer content. The double helix of DNA undergoes a reversible compaction process that favors penetration into cells and protects it from nucleases degradation. Finally, addition of the anionic surfactant to the complexes favors DNA release, allowing for a specific signal controlled release.
机译：集成的囊泡是非病毒基因转染的吸引力载体。它们具有高动力稳定性，制备程序简单且便宜。另外，通过改变组件之间的摩尔比，容易调制它们的尺寸和电荷。由于这些原因，我们研究了用小牛胸腺DNA对由DIDECYLDIMETMINOM溴（DIDAB）和8-十六烷基硫酸盐（8-SHS）制成的带正电荷的囊泡囊泡之间的相互作用。获得强烈关联复合物，其结构取决于DNA含量。在低浓度下，DNA /囊泡配合物是稳定的，具有与裸囊泡非常相似的特征。在DNA存在下，形成多层层实体;该过程是通过DNA /囊泡复合物的聚集和重排的促进。表面吸附到囊泡上与DNA含量成比例地增加。在这种条件下，Zeta潜力突然降低，因为形成了囊泡标识的大簇。此后，发生沉淀。相应地获得的固体是具有夹在Lamellae之间的DNA的层状相。层状相和沉淀组成中DNA分子之间的D-1距离取决于生物聚合物含量。 DNA的双螺旋经历了可逆压实过程，使渗透到细胞中并保护其免受核酸酶降解。最后，向复合物中加入阴离子表面活性剂，优于DNA释放，允许特定的信号控制释放。

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  • 来源
    《RSC Advances》 |2015年第99期|共8页
  • 作者

    Pucci C.; Scipioni A.; Diociaiuti M.; La Mesa C.; Perez L.; Pons R.;

  • 作者单位

    Univ Roma La Sapienza Dept Chem I-00185 Rome Italy;

    Univ Roma La Sapienza Dept Chem I-00185 Rome Italy;

    Ist Super Sanita Dip Tecnol &

    Salute I-00185 Rome Italy;

    Univ Roma La Sapienza Dept Chem I-00185 Rome Italy;

    IQAC CSIC Inst Quim Avancada Catalunya Barcelona 08034 Spain;

    IQAC CSIC Inst Quim Avancada Catalunya Barcelona 08034 Spain;

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  • 正文语种 eng
  • 中图分类 化学;
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