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首页> 外文期刊>Acta biomaterialia >Effect of calcium phosphate coating and rhBMP-2 on bone regeneration in rabbit calvaria using poly(propylene fumarate) scaffolds
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Effect of calcium phosphate coating and rhBMP-2 on bone regeneration in rabbit calvaria using poly(propylene fumarate) scaffolds

机译：聚富马酸丙二醇酯支架磷酸钙涂层和rhBMP-2对兔颅骨骨再生的影响

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Various calcium phosphate based coatings have been evaluated for better bony integration of metallic implants and are currently being investigated to improve the surface bioactivity of polymeric scaffolds. The aim of this study was to evaluate the role of calcium phosphate coating and simultaneous delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2) on the in vivo bone regeneration capacity of biodegradable, porous poly(propylene fumarate) (PPF) scaffolds. PPF scaffolds were coated with three different calcium phosphate formulations: magnesium-substituted beta-tricalcium phosphate (beta-TCMP), carbonated hydroxyapatite (synthetic bone mineral, SBM) and biphasic calcium phosphate (BCP). In vivo bone regeneration was evaluated by implantation of scaffolds in a critical-sized rabbit calvarial defect loaded with different doses of rhBMP-2. Our data demonstrated that scaffolds with each of the calcium phosphate coatings were capable of sustaining rhBMP-2 release and retained an open porous structure. After 6 weeks of implantation, micro-computed tomography revealed that the rhBMP-2 dose had a significant effect on bone formation within the scaffolds and that the SBM-coated scaffolds regenerated significantly greater bone than BCP-coated scaffolds. Mechanical testing of the defects also indicated restoration of strength in the SBM and beta-TCMP with rhBMP-2 delivery. Histology results demonstrated bone growth immediately adjacent to the scaffold surface, indicating good osteointegration and osteoconductivity for coated scaffolds. The results obtained in this study suggest that the coated scaffold platform demonstrated a synergistic effect between calcium phosphate coatings and rhBMP-2 delivery and may provide a promising platform for the functional restoration of large bone defects. (C) 2015 Published by Elsevier Ltd. on behalf of Acta Materialia Inc.
机译：已经评估了各种基于磷酸钙的涂层以更好地金属植入物的骨整合，并且目前正在研究以改善聚合物支架的表面生物活性。这项研究的目的是评估磷酸钙涂层和同时传递重组人骨形态发生蛋白2（rhBMP-2）对可生物降解的多孔聚富马酸丙二酯（PPF）支架的体内骨再生能力的作用。 。 PPF支架涂有三种不同的磷酸钙制剂：镁取代的β-磷酸三钙（β-TCMP），碳酸羟基磷灰石（合成骨矿物质，SBM）和双相磷酸钙（BCP）。通过将支架植入具有不同剂量的rhBMP-2的临界大小的兔颅盖缺损中，评估了体内的骨再生。我们的数据表明具有每个磷酸钙涂层的支架能够维持rhBMP-2的释放并保持开放的多孔结构。植入6周后，显微计算机断层扫描显示，rhBMP-2剂量对支架内的骨形成有显着影响，而SBM涂覆的支架比BCP涂覆的支架再生的骨骼明显更大。缺陷的机械测试还表明，随着rhBMP-2的传递，SBM和β-TCMP的强度得以恢复。组织学结果表明紧邻支架表面的骨骼生长，表明涂层支架具有良好的骨整合性和骨传导性。在这项研究中获得的结果表明，涂​​层的支架平台显示了磷酸钙涂层和rhBMP-2传递之间的协同作用，并可能为大骨缺损的功能修复提供有希望的平台。 （C）2015由Elsevier Ltd.代表Acta Materialia Inc.发布。

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