Cytogenetic Characterization of the TM4 Mouse Sertoli Cell Line. II. Chromosome Microdissection, FISH, Scanning Electron Microscopy, and Confocal Laser Scanning Microscopy

Schmid Michael; Guttenbach Martina; Steinlein Claus; Wanner Gerhard; Houben Andreas

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Cytogenetic Characterization of the TM4 Mouse Sertoli Cell Line. II. Chromosome Microdissection, FISH, Scanning Electron Microscopy, and Confocal Laser Scanning Microscopy

机译：TM4小鼠支持细胞系的细胞遗传学表征。二。染色体显微切割，FISH，扫描电子显微镜和共聚焦激光扫描显微镜

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The chromosomes and interphase cell nuclei of the permanent mouse Sertoli cell line TM4 were examined by chromosome microdissection, FISH, scanning electron microscopy, and confocal laser scanning microscopy. The already known marker chromosomes m(1)-m(5) were confirmed, and 2 new large marker chromosomes m6 and m7 were characterized. The minute heterochromatic marker chromosomes m(4) and m(5) were microdissected and their DNA amplified by DOP-PCR. FISH of this DNA probe on TM4 metaphase chromosomes demonstrated that the m(4) and m(5) marker chromosomes have derived from the centromeric regions of normal telocentric mouse chromosomes. Ectopic pairing of the m(4) and m(5) marker chromosomes with the centromeric region of any of the other chromosomes (centromeric associations) was apparent in similar to 60% of the metaphases. Scanning electron microscopy revealed DNA-protein bridges connecting the centromeric regions of normal chromosomes and the associated m(4) and m(5) marker chromosomes. Interphase cell nuclei of TM4 Sertoli cells did not exhibit the characteristic mor-phology of Sertoli cells in the testes of adult mice as shown by fluorescence microscopy and confocal laser scanning microscopy. (C) 2016 S. Karger AG, Basel

机译：通过染色体显微切割，FISH，扫描电子显微镜和共聚焦激光扫描显微镜检查永久小鼠Sertoli细胞系TM4的染色体和相间细胞核。确认了已知的标记染色体m（1）-m（5），并鉴定了2个新的大标记染色体m6和m7。显微切割微小的异色标记染色体m（4）和m（5），并通过DOP-PCR扩增其DNA。在TM4中期染色体上的该DNA探针的FISH表明，m（4）和m（5）标记染色体已源自正常端粒小鼠染色体的着丝粒区域。 m（4）和m（5）标记染色体与任何其他染色体的着丝粒区域（着丝粒关联）的异位配对在大约60％的中期都很明显。扫描电子显微镜显示DNA-蛋白质桥连接正常染色体的着丝粒区域和相关的m（4）和m（5）标记染色体。荧光显微镜和共聚焦激光扫描显微镜显示，成年小鼠睾丸中TM4 Sertoli细胞的间期细胞核未表现出Sertoli细胞的特征形态。（C）2016 S.Karger AG，巴塞尔

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《Cytogenetic and genome research》 |2015年第3期|共9页
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Schmid Michael; Guttenbach Martina; Steinlein Claus; Wanner Gerhard; Houben Andreas;
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正文语种 eng
中图分类普通生物学;
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Electron microscopy; FISH; Marker chromosomes; Microdissection; Mouse Sertoli cell line; TM4;

机译：电子显微镜;FISH;标记染色体;显微切割;小鼠支持细胞;TM4;
入库时间 2022-08-18 09:49:46

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机译：TM4小鼠支持细胞系的细胞遗传学表征。二。染色体显微切割，FISH，扫描电子显微镜和共聚焦激光扫描显微镜
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Cytogenetic Characterization of the TM4 Mouse Sertoli Cell Line. II. Chromosome Microdissection, FISH, Scanning Electron Microscopy, and Confocal Laser Scanning Microscopy

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