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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray diffraction studies of cyanuric acid hydrolase from Azorhizobium caulinodans
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Crystallization and preliminary X-ray diffraction studies of cyanuric acid hydrolase from Azorhizobium caulinodans

机译：菜青虫中氰尿酸水解酶的结晶和初步X射线衍射研究

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摘要

Cyanuric acid is synthesized industrially and forms during the microbial metabolism of s-triazine herbicides. Cyanuric acid is metabolized by some microorganisms via cyanuric acid hydrolase (CAH), which opens the s-triazine ring as a prelude to further metabolism. CAH is a member of the rare cyanuric acid hydrolase/barbiturase family. Here, the crystallization and preliminary X-ray diffraction analysis of CAH from Azorhizobium caulinodans are reported. CAH was cocrystallized with barbituric acid, a close analog of cyanuric acid that is a tight-binding competitive inhibitor. Crystals suitable for X-ray diffraction experiments were grown in conditions containing PEG 8K or magnesium sulfate as precipitants. An X-ray diffraction data set was collected from CAH-barbituric acid crystals to 2.7 angstrom resolution. The crystals were found to belong to space group I4(1)22, with unit-cell parameters a = b = 237.9, c = 105.3 angstrom, alpha = beta = gamma = 90 degrees.
机译：工业上合成的氰尿酸是在S-三嗪除草剂的微生物代谢过程中形成的。某些微生物通过氰尿酸水解酶（CAH）代谢氰尿酸，该酶打开s-三嗪环，作为进一步代谢的序幕。 CAH是稀有的氰尿酸水解酶/巴比妥酶家族的成员。在此，报道了从阿魏根瘤菌藻类中CAH的结晶和初步X射线衍射分析。 CAH与巴比妥酸共结晶，巴比妥酸是氰尿酸的紧密类似物，是紧密结合的竞争性抑制剂。适用于X射线衍射实验的晶体在含有PEG 8K或硫酸镁作为沉淀剂的条件下生长。从CAH-巴比妥酸晶体收集到2.7埃分辨率的X射线衍射数据集。发现该晶体属于空间群I4（1）22，其晶胞参数a = b = 237.9，c = 105.3埃，α=β=γ= 90度。

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