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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary X-ray crystallographic studies of DnaJ from Streptococcus pneumoniae
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Crystallization and preliminary X-ray crystallographic studies of DnaJ from Streptococcus pneumoniae

机译：肺炎链球菌DnaJ的结晶和初步X射线晶体学研究

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摘要

DnaJ, cooperating with DnaK and GrpE, promotes the folding of unfolded hydrophobic polypeptides, dissociates protein complexes and translocates protein across membranes. Additionally, DnaJ from Streptococcus pneumoniae (SpDnaJ) is involved in the infectious disease process and is being developed as a potential vaccine to prevent bacterial infection. Here the expression, purification, crystallization and preliminary crystallographic analysis of SpDnaJ are reported. The crystals belong to space groups I222 or I212121 and the diffraction resolution is 3.0 angstrom with unit-cell parameters a = 47.68, b = 104.45, c = 234.57 angstrom. The crystal most likely contains one molecule in the asymmetric unit, with a VM value of 3.24 angstrom 3Da1 and a solvent content of 62.1%.
机译：DnaJ与DnaK和GrpE合作，促进未折叠疏水多肽的折叠，解离蛋白质复合物并跨膜转运蛋白质。另外，来自肺炎链球菌的DnaJ（SpDnaJ）参与了传染病的过程，并正在开发为预防细菌感染的潜在疫苗。这里报道了SpDnaJ的表达，纯化，结晶和初步晶体学分析。晶体属于I222或I212121空间群，其衍射分辨率为3.0埃，晶胞参数a = 47.68，b = 104.45，c = 234.57埃。该晶体很可能在不对称单元中包含一个分子，VM值为3.24埃3Da1，溶剂含量为62.1％。

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