首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex
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Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex

机译:4-硫尿苷合成酶-RNA复合物的纯化,结晶和初步晶体学分析

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摘要

The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 angstrom. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 angstrom.
机译:硫转移酶4-硫尿苷合成酶(ThiI)参与了tRNA中U8的ATP依赖性修饰。克隆,过表达和纯化了来自滨海嗜热菌的ThiI。制备并结晶包含ThiI和截短的tRNA的复合物,并收集X射线衍射数据至3.5埃的分辨率。晶体属于正交晶体空间群P2(1)2(1)2(1),其晶胞参数a = 102.9,b = 112.8,c = 132.8埃。

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