• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Structure of PatF from Prochloron didemni
【24h】

Structure of PatF from Prochloron didemni

机译：Prochloron didemni的PatF的结构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein.
机译：patellamides是具有强力生物学效应的大环肽，是cyanactactins的子集。蓝细菌素是通过一系列酶促转化产生的天然产物，常见的修饰是异戊二烯基的添加。令人费解的是，Prochloron didemni中的tell酰胺途径包含一个与早乳素有同源性的基因patF，但pa酰胺本身并未被戊烯化。克隆，表达，纯化和确定蛋白PatF的结构。无法证明该蛋白质的异戊二烯酶活性，对结构的检查揭示了该活性位点侧链同一性的变化。建议这些改变使蛋白质失活。试图突变这些残基导致未折叠的蛋白质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号