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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Use of europium ions for SAD phasing of lysozyme at the Cu K alpha wavelength
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Use of europium ions for SAD phasing of lysozyme at the Cu K alpha wavelength

机译：K离子在Cu K alpha波长用于溶菌酶的SAD定相

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摘要

Europium is shown to be a good anomalous scatterer in SAD phasing for solving the structure of biological macromolecules. The large value of the anomalous contribution of europium, f '' = 11.17 e(-), at the Cu K alpha wavelength is an advantage in de novo phasing and automated model building. Tetragonal crystals of hen egg-white lysozyme (HEWL) incorporating europium(III) chloride (50 mM) were obtained which diffracted to a resolution of 2.3 angstrom at a wavelength of 1.54 angstrom (Cu K alpha). The master data set (360 degrees frames) was split and analyzed for anomalous signal-to-noise ratio, multiplicity, completeness, SAD phasing and automated building. The structure solution and model building of the split data sets were carried out using phenix.autosol and phenix.autobuild. The contributions of the Eu ions to SAD phasing using in-house data collection are discussed. This study revealed successful lysozyme phasing by SAD using laboratory-source data involving Eu ions, which are mainly coordinated by the side chains of Asn46, Asp52 and Asp101 together with some water molecules.
机译：在解决生物大分子结构的SAD阶段中，is是一种很好的反常散射体。在Cu Kα波长处，f的异常贡献f''= 11.17 e（-）的较大值是从头定相和自动模型构建的一个优势。获得掺入氯化euro（III）（50mM）的鸡蛋清溶菌酶（HEWL）的四方晶体，其在1.54埃（Cu Kα）的波长下衍射至2.3埃的分辨率。拆分主数据集（360度帧），并分析异常信噪比，多重性，完整性，SAD相位调整和自动构建。使用phenix.autosol和phenix.autobuild进行拆分数据集的结构求解和模型构建。讨论了使用内部数据收集的Eu离子对SAD定相的贡献。这项研究揭示了利用涉及Eu离子的实验室来源数据成功地通过SAD进行溶菌酶定相，这些数据主要由Asn46，Asp52和Asp101的侧链以及一些水分子协调。

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