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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 angstrom resolution
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Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 angstrom resolution

机译：酸性嗜酸纤维分解酶家族74糖苷水解酶的结构，分辨率为1.82埃

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摘要

Here, a 1.82 angstrom resolution X-ray structure of a glycoside hydrolase family 74 (GH74) enzyme from Acidothermus cellulolyticus is reported. The resulting structure was refined to an R factor of 0.150 and an R-free of 0.196. Structural analysis shows that five related structures have been reported with a secondary-structure similarity of between 75 and 89%. The five similar structures were all either Clostridium thermocellum or Geotrichum sp. M128 GH74 xyloglucanases. Structural analysis indicates that the A. cellulolyticus GH74 enzyme is an endoxyloglucanase, as it lacks a characteristic loop that blocks one end of the active site in exoxyloglucanases. Superimposition with the C. thermocellum GH74 shows that Asp451 and Asp38 are the catalytic residues.
机译：在此，报道了来自解酸嗜酸菌的糖苷水解酶家族74（GH74）酶的1.82埃分辨率的X射线结构。将所得结构精制至R因子为0.150和无R值为0.196。结构分析表明，已经报道了五种相关结构，其二级结构相似性在75％到89％之间。五个相似的结构都是热纤梭菌或Geotrichum sp。 M128 GH74木葡聚糖酶。结构分析表明，解纤维素曲霉GH74酶是一种内切葡聚糖酶，因为它缺乏阻断外切葡聚糖酶中活性位点一端的特征环。与热纤梭菌GH74的叠加显示Asp451和Asp38是催化残基。

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