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首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501
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Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501

机译：革兰氏阳性共轭质粒pIP501中转移蛋白TraM的结晶和初步结构测定

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摘要

The major means of horizontal gene spread (e. g. of antibiotic resistance) is conjugative plasmid transfer. It presents a serious threat especially for hospitalized and immuno-suppressed patients, as it can lead to the accelerated spread of bacteria with multiple antibiotic resistances. Detailed information about the process is available only for bacteria of Gram-negative (G-) origin and little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. Here we present the purification, biophysical characterization, crystallization and preliminary structure determination of the TraM C-terminal domain (TraM Delta, comprising residues 190-322 of the full-length protein), a putative transfer protein from the G+ conjugative model plasmid pIP501. The crystals diffracted to 2.5 angstrom resolution and belonged to space group P1, with unit-cell parameters a = 39.21, b = 54.98, c = 93.47 angstrom, alpha = 89.91, beta = 86.44, gamma = 78.63 degrees and six molecules per asymmetric unit. The preliminary structure was solved by selenomethionine single-wavelength anomalous diffraction.
机译：水平基因传播的主要手段（例如抗生素抗性）是结合质粒转移。它尤其对住院和免疫抑制的患者构成严重威胁，因为它可以导致具有多种抗生素抗性的细菌加速传播。有关此过程的详细信息仅适用于革兰氏阴性（G-）来源的细菌，而对革兰氏阳性（G +）细菌的相应机制知之甚少。在这里，我们介绍了TraM C末端结构域（TraM Delta，包括全长蛋白的190-322位残基）的纯化，生物物理表征，结晶和初步结构测定，这是一种从G +结合模型质粒pIP501推定的转移蛋白。晶体衍射到2.5埃分辨率，属于空间群P1，单位晶胞参数a = 39.21，b = 54.98，c = 93.47埃，α= 89.91，β= 86.44，γ= 78.63度，每个不对称单元有六个分子。通过硒代蛋氨酸单波长异常衍射解决了初步结构。

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