Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans

Yue Yang; Liu Shengquan; Li Hongbin; Song Binghong; Xie Ting; Sun Yan; Chao Yapeng; Qian Shijun

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Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans

机译：Macerans芽孢杆菌Tyr167His突变体α-环糊精葡糖基转移酶的结晶和初步X射线衍射研究

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Improving the specificity of alpha-cyclodextrin glucanotransferase is a significant issue in the field of alpha-cyclodextrin production. In this study, a constructed Y167H mutant alpha-cyclodextrin glucanotransferase with enhanced alpha-cyclodextrin specificity was successfully expressed and purified. Single crystals were grown using PEG 4000 as a precipitating agent by the hanging-drop vapour-diffusion method at 293 K. The crystals exhibited two kinds of morphology in different crystallization conditions. The crystals diffracted to at least 2.2 A resolution (space group P2(1)2(1)2(1)), with unit-cell parameters a = 65.69, b = 78.70, c = 137.00 angstrom. Assuming the asymmetric cell to be occupied by a monomer of 75 kDa, the unit cell contains 43.77% solvent with a crystal volume per protein mass, V-M, of 2.19 angstrom(3) Da(-1)

机译：改进α-环糊精葡聚糖转移酶的特异性是α-环糊精生产领域中的重要问题。在这项研究中，成功地表达和纯化了构建的具有增强的α-环糊精特异性的Y167H突变体α-环糊精葡聚糖转移酶。使用PEG 4000作为沉淀剂，通过滴滴蒸汽扩散法在293 K下生长单晶。在不同的结晶条件下，该晶体表现出两种形态。晶体衍射到至少2.2 A的分辨率（空间群P2（1）2（1）2（1）），晶胞参数a = 65.69，b = 78.70，c = 137.00埃。假设不对称细胞被75 kDa的单体占据，该晶胞包含43.77％的溶剂，每蛋白质质量的晶体体积V-M为2.19埃（3）Da（-1）。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第10期|共4页
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Yue Yang; Liu Shengquan; Li Hongbin; Song Binghong; Xie Ting; Sun Yan; Chao Yapeng; Qian Shijun;
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1. Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans [J] . Yue Yang, Liu Shengquan, Li Hongbin, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第10期

机译：Macerans芽孢杆菌Tyr167His突变体α-环糊精葡糖基转移酶的结晶和初步X射线衍射研究
2. Crystallization and preliminary X-ray diffraction studies of HutP protein: an RNA-binding protein that regulates the transcription of hut operon in Bacillus subtilis [J] . Kumarevel TS., Fujimoto Z., Padmanabhan B., Journal of Structural Biology . 2002,第3期

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6. Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant α-cyclodextrin glucanotransferase from Bacillus macerans [O] . Yang Yue, Shengquan Liu, Hongbin Li, 2013

机译：Macerans芽孢杆菌Tyr167His突变体α-环糊精葡聚糖转移酶的结晶和初步X射线衍射研究
7. Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant α-cyclodextrin glucanotransferase fromBacillus macerans [O] . Yang Yue, Shengquan Liu, Hongbin Li, 2013

机译：TYR167HIS突变体α-环糊精葡聚糖转移酶的结晶和初步X射线衍射研究

Crystallization and preliminary X-ray diffraction studies of Tyr167His mutant alpha-cyclodextrin glucanotransferase from Bacillus macerans

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