Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae

Zhang Caizhi; Liu Xinqi; Qiu Dewen; Zeng Hongmei

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Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae

机译：稻瘟病菌效应蛋白MoHrip1的纯化，结晶和初步X射线衍射分析

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The effector protein MoHrip1 from the pathogenic fungus Magnaporthe oryzae was purified and crystallized using the sitting-drop vapour-diffusion method. Native crystals appeared in a solution composed of 0.005 M cobalt(II) chloride hexahydrate, 0.005 M nickel(II) chloride hexahydrate, 0.005 M cadmium chloride hydrate, 0.005 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 12%(w/v) polyethylene glycol 3350. A native data set was collected to 1.9 angstrom resolution at 100 K using an in-house X-ray source. The structure of MoHrip1 was successfully determined by molecular replacement using a homologous structure.

机译：使用坐滴蒸气扩散法纯化和结晶来自病原真菌米格霉的效应蛋白MoHrip1。天然晶体出现在由0.005 M六水合氯化钴（II），0.005 M六水合氯化镍（II），0.005 M水合氯化镉，0.005 M四水合氯化镁，0.1 M HEPES pH 7.5、12％（w / v）组成的溶液中）聚乙二醇3350。使用内部X射线源在100 K下以1.9埃的分辨率收集了一个原始数据集。 MoHrip1的结构已通过使用同源结构的分子置换成功确定。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第4期|共3页
作者
Zhang Caizhi; Liu Xinqi; Qiu Dewen; Zeng Hongmei;
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1. Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae [J] . Zhang Caizhi, Liu Xinqi, Qiu Dewen, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第4期

机译：稻瘟病菌效应蛋白MoHrip1的纯化，结晶和初步X射线衍射分析
2. Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae [J] . Liu Mengjie, Liu Xinqi, Zeng Hongmei, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第4期

机译：稻瘟病菌效应蛋白MoHrip2的纯化，结晶及X射线初步分析
3. Expression, purification, crystallization and preliminary X-ray diffraction analysis of the effector-interaction domain of the resistance protein RGA5-A from Oryza sativa L. japonica [J] . Huang Dan, Zhang Yanan, Zhao Yanxiang, Acta crystallographica. Section F, Structural biology communications . 2015,第2期

机译：水稻抗性蛋白RGA5-A的效应子-相互作用域的表达，纯化，结晶和X射线初步分析
4. PURIFICATION AND CHARACTERIZATION OF A PROTEINACEOUS ELICITOR FROM XANTHOMONAS ORYZAE PV. ORYZAE [C] . Weigang WEN Xuzhong LU, Jingsheng WANG Asian Conference on Plant Pathology . 2001

机译：Xanthomonas oryzae PV的蛋白质化学者的纯化和表征。 oryzae.
5. Stringent starvation protein A: Expression, purification, characterization, and preliminary x-ray diffraction studies [D] . Andrykovitch, Michelle 2002

机译：严格的饥饿蛋白A：表达，纯化，表征和初步X射线衍射研究
6. Purification crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae [O] . Caizhi Zhang, Xinqi Liu, Dewen Qiu, 2013

机译：稻瘟病菌效应蛋白MoHrip1的纯化结晶和初步X射线衍射分析
7. Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 fromMagnaporthe oryzae [O] . Mengjie Liu, Xinqi Liu, Hongmei Zeng, 2013

机译：效应蛋白Mohrip2的纯化，结晶和初步X射线衍射分析来自magnaporthe oryzae

Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae

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