Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae

Liu Mengjie; Liu Xinqi; Zeng Hongmei; Qiu Dewen

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Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae

机译：稻瘟病菌效应蛋白MoHrip2的纯化，结晶及X射线初步分析

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MoHrip2, a novel effector protein from the pathogenic fungus Magnaporthe oryzae, was purified and crystallized using the sitting-drop vapour-diffusion method. Native crystals and selenomethionine-labelled crystals were obtained using 2.2 M ammonium sulfate as a precipitant. A native data set was collected to 2.0 angstrom resolution at 100 K using an in-house X-ray source and a selenomethionine-labelled data set containing anomalous signal was collected to 1.8 angstrom resolution at 100 K using a synchrotron source. Based on the anomalous signal generated from the Se atom, the MoHrip2 structure was successfully solved using the single-wavelength anomalous dispersion (SAD) method.

机译：MoHrip2是一种来自病原真菌米格纳霉菌的新型效应蛋白，使用坐滴蒸汽扩散法纯化和结晶。使用2.2 M硫酸铵作为沉淀剂可得到天然晶体和硒甲硫氨酸标记的晶体。使用内部X射线源在100 K下将原始数据集收集到2.0埃分辨率，并使用同步加速器源在100 K下将包含异常信号的硒代蛋氨酸标记的数据集收集到1.8埃分辨率。基于硒原子产生的异常信号，使用单波长异常色散（SAD）方法成功地解决了MoHrip2结构。

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《Acta crystallographica, Section F. Structural biology and crystallization communications》 |2013年第4期|共5页
作者
Liu Mengjie; Liu Xinqi; Zeng Hongmei; Qiu Dewen;
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1. Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae [J] . Liu Mengjie, Liu Xinqi, Zeng Hongmei, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第4期

机译：稻瘟病菌效应蛋白MoHrip2的纯化，结晶及X射线初步分析
2. Purification, crystallization and preliminary X-ray diffraction analysis of the effector protein MoHrip1 from Magnaporthe oryzae [J] . Zhang Caizhi, Liu Xinqi, Qiu Dewen, Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第4期

机译：稻瘟病菌效应蛋白MoHrip1的纯化，结晶和初步X射线衍射分析
3. Expression, purification, crystallization and preliminary X-ray diffraction analysis of the effector-interaction domain of the resistance protein RGA5-A from Oryza sativa L. japonica [J] . Huang Dan, Zhang Yanan, Zhao Yanxiang, Acta crystallographica. Section F, Structural biology communications . 2015,第2期

机译：水稻抗性蛋白RGA5-A的效应子-相互作用域的表达，纯化，结晶和X射线初步分析
4. PURIFICATION AND CHARACTERIZATION OF A PROTEINACEOUS ELICITOR FROM XANTHOMONAS ORYZAE PV. ORYZAE [C] . Weigang WEN Xuzhong LU, Jingsheng WANG Asian Conference on Plant Pathology . 2001

机译：Xanthomonas oryzae PV的蛋白质化学者的纯化和表征。 oryzae.
5. Stringent starvation protein A: Expression, purification, characterization, and preliminary x-ray diffraction studies [D] . Andrykovitch, Michelle 2002

机译：严格的饥饿蛋白A：表达，纯化，表征和初步X射线衍射研究
6. Purification crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae [O] . Mengjie Liu, Xinqi Liu, Hongmei Zeng, 2013

机译：稻瘟病菌效应蛋白MoHrip2的纯化结晶及X射线初步分析
7. Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 fromMagnaporthe oryzae [O] . Mengjie Liu, Xinqi Liu, Hongmei Zeng, 2013

机译：效应蛋白Mohrip2的纯化，结晶和初步X射线衍射分析来自magnaporthe oryzae

Purification, crystallization and preliminary X-ray diffraction analysis of effector protein MoHrip2 from Magnaporthe oryzae

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