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In vitro evaluation of functionalized decellularized muscle scaffold for in situ skeletal muscle regeneration

机译：用于原位骨骼肌再生的官能化脱细胞肌脚手架的体外评价

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摘要

Current treatment options for repairing volumetric muscle loss injury involve the use of existing host tissue like muscular flaps or grafts. However, host muscle tissue may not be available and donor site morbidity, such as functional loss and volume deficiency, is often present. In this study, we developed a biofunctionalized muscle-derived decellularized extracellular matrix scaffolding system to utilize endogenous stem/progenitor cells for in situ muscle tissue regeneration.Weoptimized the decellularization process to enhance cellular infiltration and fabricated an insulin-like growth factorbinding protein 3 (IGFBP-3)-conjugated scaffold for controlled delivery of IGF-I.Wethen tested in vitro characterization including IGF-I release kinetics and cellular infiltration. In addition, we have analyzed the bioactivities of skeletal muscle cells (C2C12) to assess the indirect effect of released IGF-1 from the scaffold. The IGFBP-3 conjugated scaffolds demonstrated showed sustained release of IGF-1 and1%SDS decellularized scaffold with IGF-1 showed higher cellular infiltration compared to control scaffolds (no conjugation). In indirect bioactivity assay, IGF-1 conjugated scaffold showed 2.1-fold increased cell activity compared to control (fresh media). Our results indicate that IGFBP-3/ IGF-I conjugated scaffold has the potential to be used for in situ muscle tissue regeneration.
机译：用于修复体积肌损伤损伤的目前的治疗方案涉及使用现有的宿主组织，如肌肉襟翼或移植物。然而，宿主组织可能无法获得，并且通常存在诸如功能性损失和体积缺乏的供体部位发病率。在这项研究中，我们开发了一种生物功能化肌肉衍生的脱细胞外基质基质脚手架系统，用于利用内源性茎/祖细胞用于原位肌肉组织再生。优化脱细胞化过程以增强细胞浸润并制造胰岛素样生长诱导蛋白3（IGFBP -3）用于受控递送的缀合的支架IGF-I.在体外表征中测试，包括IGF-I释放动力学和细胞浸润。此外，我们已经分析了骨骼肌细胞（C2C12）的生物活像，以评估来自支架的释放IGF-1的间接效果。所证明的IGFBP-3缀合支架显示出持续释放IGF-1和1％SDS脱细胞的支架，其与控制支架（无缀合）相比显示出更高的细胞浸润。在间接生物活性测定中，与对照（新鲜培养基）相比，IGF-1共轭支架显示出2.1倍的细胞活性。我们的结果表明IGFBP-3 / IGF-I缀合的支架具有用于原位肌肉组织再生的可能性。

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  • 来源
    《Biomedical materials》 |2019年第4期|共9页
  • 作者

    Lindsey Shapiro; Ebrahim Elsangeedy; Hyeongjin Lee; Anthony Atala; James J Yoo; Sang Jin Lee; Young Min Ju;

  • 作者单位

    Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine Medical Center Boulevard Winston-Salem NC27157 United States of America;

    Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine Medical Center Boulevard Winston-Salem NC27157 United States of America;

    Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine Medical Center Boulevard Winston-Salem NC27157 United States of America;

    Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine Medical Center Boulevard Winston-Salem NC27157 United States of America;

    Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine Medical Center Boulevard Winston-Salem NC27157 United States of America;

    Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine Medical Center Boulevard Winston-Salem NC27157 United States of America;

    Wake Forest Institute for Regenerative Medicine Wake Forest School of Medicine Medical Center Boulevard Winston-Salem NC27157 United States of America;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用一般科学;
  • 关键词

    skeletal muscle tissue regeneration; decellularized scaffold; IGF-I; IGFBP-3; biomaterials; tissue engineering;

    机译：骨骼肌组织再生;脱细胞化支架;IGF-I;IGFBP-3;生物材料;组织工程;

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