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Optoelectrofluidic printing system for fabricating hydrogel sheets with on-demand patterned cells and microparticles

机译：用于制造水凝胶板的光电流体印刷系统，随需按需图案化细胞和微粒

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摘要

This paper presents a novel optoelectrofluidic printing system that facilitates not only the optoelectrofluidic patterning of microparticles and mammalian cells but also the harvesting of the patterned microparticles encapsulated within poly(ethyleneglycol) dicarylate (PEGDA) hydrogel sheets. Although optoelectrofluidic technology has numerous advantages for programmable and on-demand patterning and the feasibility of manipulating single microparticles, practical applications using existing laboratory infrastructure in biological and clinical research fields have been strictly restricted due to the impossibility of recovering the final patterned product. In order to address these harvesting limitations, PEGDA was employed to utilize optoelectrofluidic printing. The Clausius-Mossotti factor was calculated to investigate the dielectrophoretic mobility of the microparticle and the cell in the PEGDA precursor solution. As a proof of concept, three basic controllabilities of the optoelectrofluidic printing system were characterized: the number of microparticles, the distance between the microparticle columns, and the ratio of two different microparticles. Furthermore, the optoelectrofluidic patterning and printing of human liver carcinoma cells (HepG2) were demonstrated in 5 min with a single-cell level of resolution. The appropriate ranges of frequency were experimentally defined based on the calculated result of the dielectrophoretic mobility of HepG2 cells. Finally, optoelectrofluidically cell-patterned hydrogel sheets were successfully recovered under a highly viable physiological condition.
机译：本文介绍了一种新型光电流制印刷系统，不仅便于微粒和哺乳动物细胞的光电流体图案化，而且促进了包封在聚（乙二醇）二亚芳酯（PEGDA）水凝胶片中的图案化的微粒的收获。尽管光电流体技术具有许多可编程和按需图案的优势，但操作单片机的可行性，由于恢复最终图案产品的不可能性，严格限制了使用生物和临床研究领域的现有实验室基础设施的实际应用。为了解决这些收获限制，使用PEGDA来利用光电流体印刷。计算克劳斯·莫斯托因子以研究PEGDA前体溶液中微粒和细胞的介电电泳迁移率。作为概念证据，表征了光电流体印刷系统的三种基本控制性：微粒的数量，微粒柱之间的距离，以及两种不同的微粒的比例。此外，在5分钟内用单细胞分辨率进行5分钟对人肝癌细胞（HepG2）的光电流体图案化和印刷。基于HepG2细胞的介电电泳迁移率的计算结果，实验地定义了适当的频率范围。最后，在高度可行的生理条件下成功回收光电流过细胞图案化水凝胶片。

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  • 来源
    《Biofabrication》 |2017年第1期|共9页
  • 作者

    Hyun Ji Gi; Dongsik Han; Je-Kyun Park;

  • 作者单位

    Department of Bio and Brain Engineering Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-ro Yuseong-gu Daejeon 34141 Republic of Korea;

    Department of Bio and Brain Engineering Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-ro Yuseong-gu Daejeon 34141 Republic of Korea;

    Department of Bio and Brain Engineering Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-ro Yuseong-gu Daejeon 34141 Republic of Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;
  • 关键词

    dielectrophoresis; hydrogel sheet; on-demand patterning; optoelectrofluidics;

    机译：介电电泳;水凝胶板;按需图案化;光电流体;

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