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Biofabrication of bacterial nanocellulose scaffolds with complexvascular structure

机译：具有复合血管结构的细菌纳米纤维素支架的生物制造

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Bacterial nanocellulose (BNC) has proven to be an effective hydrogel-like material for different tissueengineering applications due to its biocompatibility and good mechanical properties. However, as forall biomaterials, in vitro biosynthesis of large tissue constructs remains challenging due to insufficientoxygen and nutrient transport in engineered scaffold-cell matrices. In this study we designed,biofabricated and evaluated bacterial nanocellulose scaffolds with a complex vascular mimetic lumenstructure. As a first step a method for creating straight channeled structures within a bacterialnanocellulose scaffold was developed and evaluated by culturing of Human Umbilical VeinEndothelial Cells(HUVECs). In a second step, more complex structures within the scaffolds wereproduced utilizing a 3D printer. A print mimicking a vascular tree acted as a sacrificial template toproduce a network within the nanoporous bacterial nanocellulose scaffolds that could be lined withendothelial cells. In a last step, a method to produce large constructs with interconnected macroporosity and vascular like lumen structure was developed. In this process patient data from x-raycomputed tomography scans was used to create a mold for casting a full-sized kidney construct. Byshowing that the 3D printing technology can be combined with BNC biosynthesis we hope to widenthe opportunities of 3D printing, while also enabling the production of BNC scaffolds constructs withtailored vascular architectures and properties.
机译：由于其生物相容性和良好的机械性能，已被证明是一种用于不同疗养学应用的有效水凝胶状材料。然而，由于持续的生物材料，大型组织构建体的体外生物合成仍然是由于工程支架细胞基质中不足的氧约和营养转运而挑战。在这项研究中，我们设计了与复杂的血管模拟腔结构设计，生物伪造和评估的细菌纳米纤维素支架。作为第一步骤，通过培养人脐脉细胞（HUVEC），开发并评估并评估用于在细菌碱纤维素支架中产生直流结构的方法。在第二步中，使用3D打印机的支架内的更复杂的结构。模拟血管树的印刷作为牺牲模板，拓扑纳米孔细菌纳米纤维素支架内的网络，其可以被衬有内衬细胞。在最后一步中，开发了一种用互连的大孔隙度和血管结构产生大构建体的方法。在该过程中，患者来自X射线扫描断层扫描的数据用于产生用于铸造全尺寸肾脏结构的模具。通过培养，3D打印技术可以与BNC生物合成相结合，我们希望Widthe 3D打印的机会，同时也能够生产BNC脚手架构建体，其具有血管架构和性质。

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