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Polymorphic Imprinting of SLC38A4 Gene in Bovine Placenta

机译：牛胎盘中SLC38A4基因的多态性印记

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摘要

Imprinted genes are characterized by monoallelic expression that is dependent on parental origin. Comparative analysis of imprinted genes between species is a powerful tool for understanding the biological significance of genomic imprinting. The slc38a4 gene encodes a neutral amino acid transporter and is identified as imprinted in mice. In this study, the imprinting status of SLC38A4 was assessed in bovine adult tissues and placenta using a polymorphism-based approach. Results indicate that SLC38A4 is not imprinted in eight adult bovine tissues including heart, liver, spleen, lung, kidney, muscle, fat, and brain. It was interesting to note that SLC38A4 showed polymorphic status in five heterogeneous placentas, with three exhibiting paternal monoallelic expression and two exhibiting biallelic expression. Monoallelic expression of imprinted genes is generally associated with allele-specific differentially methylation regions (DMRs) of CpG islands (CGIs)-encompassed promoter; therefore, the DNA methylation statuses of three CGIs in the SLC38A4 promoter and exon 1 region were tested in three placentas (two exhibiting paternal monoallelic and one showing biallelic expression of SLC38A4) and their corresponding paternal sperms. Unexpectedly, extreme hypomethylation (3%) of the DNA was observed in all the three detected placentas and their corresponding paternal sperms. The absence of DMR in bovine SLC38A4 promoter region implied that DNA methylation of these three CGIs does not directly or indirectly affect the polymorphic imprinting of SLC38A4 in bovine placenta. This suggested other epigenetic features other than DNA methylation are needed in regulating the imprinting of bovine SLC38A4, which is different from that of mouse with respect to a DMR existence at the mouse's slc38a4 promoter region. Although further work is needed, this first characterization of polymorphic imprinting status of SLC38A4 in cattle placenta provides valuable information on investigating the genomic imprinting phenomenon itself.
机译：被印迹基因的特征在于单独的表达，这些表达依赖于父母来源。物种之间印迹基因的比较分析是理解基因组印记的生物学意义的强大工具。 SLC38a4基因编码中性氨基酸转运蛋白，并在小鼠中鉴定为印迹。在该研究中，使用基于多态性的方法在牛成人组织和胎盘中评估SLC38A4的印迹状态。结果表明，SLC38A4在八个成人牛组织中没有印记，包括心脏，肝脏，脾脏，肺，肾，肌肉，脂肪和脑。值得注意的是，SLC38A4在五种异质胎盘中显示出多态性状态，其中具有三种表现出父族单相位表达和表现出比亚莱氏表现。印迹基因的单梯度表达通常与CpG岛（CGIS）的等位基因特异性差异甲基化区域（DMRS）相关;因此，在SLC38A4启动子和外显子1区域中的三个CGI的DNA甲基化状态在三个胎盘（两个表现出父族单链和SLC38A4的双晶体表达）中测试及其相应的父子精子。出乎意料地，在所有三种检测到的胎盘和它们相应的父子精子中观察到极端的低甲基化（＆ 3％）DNA。在牛SLC38A4启动子区域中没有DMR暗示这三种CGI的DNA甲基化不直接或间接地影响SLC38A4在牛胎盘中的多态性压印。这提出了除DNA甲基化之外的其他表观遗传特征，用于调节牛SLC38A4的压印，这与小鼠的SLC38A4启动子区域的DMR存在不同于小鼠的印迹。虽然需要进一步的工作，但是在牛胎盘中SLC38A4的多态压印状态的第一次表征提供了有关调查基因组印记现象本身的有价值的信息。

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  • 来源
    《Biochemical Genetics》 |2018年第6期|共11页
  • 作者

    Xu Da; Zhang Cui; Li Junliang; Wang Guannan; Chen Weina; Li Dongjie; Li Shijie;

  • 作者单位

    Hebei Agr Univ Coll Life Sci Dept Biochem &

    Mol Biol Baoding 071001 Peoples R China;

    Hebei Agr Univ Coll Life Sci Dept Biochem &

    Mol Biol Baoding 071001 Peoples R China;

    Hebei Agr Univ Coll Life Sci Dept Biochem &

    Mol Biol Baoding 071001 Peoples R China;

    Hebei Agr Univ Coll Life Sci Dept Biochem &

    Mol Biol Baoding 071001 Peoples R China;

    Hebei Agr Univ Coll Life Sci Dept Biochem &

    Mol Biol Baoding 071001 Peoples R China;

    Hebei Sci &

    Technol Univ Coll Life Sci &

    Life Engn Shijiazhuang 050018 Hebei Peoples R China;

    Hebei Agr Univ Coll Life Sci Dept Biochem &

    Mol Biol Baoding 071001 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    Polymorphic imprinting; SLC38A4; Bovine placenta; Methylation; CGIs;

    机译：多态性印记;SLC38A4;牛胎盘;甲基化;CGIS;

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