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首页> 外文期刊>Biochemical Genetics >Analysis of expressed sequence tags (ESTs) from Agrostis species obtained using sequence related amplified polymorphism.
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Analysis of expressed sequence tags (ESTs) from Agrostis species obtained using sequence related amplified polymorphism.

机译：使用序列相关扩增多态性获得的Agrostis物种的表达序列标签（EST）分析。

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摘要

Bentgrass (Agrostis spp.), a genus of the Poaceae family, consists of more than 200 species and is mainly used in athletic fields and golf courses. Creeping bentgrass (A. stolonifera L.) is the most commonly used species in maintaining golf courses, followed by colonial bentgrass (A. capillaris L.) and velvet bentgrass (A. canina L.). The presence and nature of sequence related amplified polymorphism (SRAP) at the cDNA level were investigated. We isolated 80 unique cDNA fragment bands from these species using 56 SRAP primer combinations. Sequence analysis of cDNA clones and analysis of putative translation products revealed that some encoded amino acid sequences were similar to proteins involved in DNA synthesis, transcription, and signal transduction. The cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene (GenBank accession no. EB812822) was also identified from velvet bentgrass, and the corresponding protein sequence is further analyzed due to its critical role in many cellular processes. The partial peptide sequence obtained was 112 amino acids long, presenting a high degree of homology to parts of the N-terminal and C-terminal regions of cytosolic phosphorylating GAPDH (GapC). The existence of common expressed sequence tags (ESTs) revealed by a minimum evolutionary dendrogram among the Agrostis ESTs indicated the usefulness of SRAP for comparative genome analysis of transcribed genes in the grass species.
机译：Bentgrass（Agrostis SPP。），Poaceae家族的属，由200多种物种组成，主要用于运动场和高尔夫球场。匍匐的Bentgrass（A. Stolonifera L.）是维持高尔夫球场最常用的物种，其次是殖民Bentgrass（A. Capillaris L.）和天鹅绒Bentgrass（A.Canina L.）。研究了CDNA水平的序列相关扩增多态性（SRAP）的存在和性质。我们使用56 SRAP底漆组合从这些物种中孤立80个独特的cDNA片段带。 CDNA克隆的序列分析和推定翻译产品的分析显示，一些编码的氨基酸序列与参与DNA合成，转录和信号转导的蛋白质相似。还鉴定了胞岩甘油醛-3-磷酸脱氢酶（GenBank加入No.Bea112822），并且通过在许多细胞过程中，进一步分析了相应的蛋白质序列。得到的部分肽序列是112个氨基酸长，呈现高度同源性与细胞溶质磷酸化GAPDH（GAPC）的N-末端和C末端区域的部分。 Agrostis EST中最小进化树状图揭示的常见表达序列标签（EST）的存在表明SRAP在草地中转录基因的比较基因组分析的有用性。

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  • 来源
    《Biochemical Genetics》 |2008年第10期|共14页
  • 作者

    Dinler G; Budak H;

  • 作者单位

    Faculty of Engineering and Natural Sciences Biological Science and Bioengineering Program Sabanci;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
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