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首页> 外文期刊>Biochemical Genetics >Discovery and Utilization of EST-SSR Marker Resource for Genetic Diversity and Population Structure Analyses of a Subtropical Bamboo, Dendrocalamus hamiltonii
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Discovery and Utilization of EST-SSR Marker Resource for Genetic Diversity and Population Structure Analyses of a Subtropical Bamboo, Dendrocalamus hamiltonii

机译：EST-SSR标记资源对亚热带竹，Dendrocalamus Hamiltonii的遗传多样性和人口结构分析的发现与利用

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摘要

Dendrocalamus hamiltonii is a giant bamboo species native to Indian subcontinent with high economic importance. Nevertheless, highly outcross nature and flowering once in decades impose severe limitation in its propagation. Identification and mixed cultivation of genetically diverse genotypes may assist successful breeding and natural recombination of desirable traits. Characterization of existing genetic diversity and population structure are indispensable for efficient implementation of such strategies, which is facing a major challenge due to non-availability of sequence-based markers for the species. In this study, 8121 EST-SSR markers were mined from D. hamiltonii transcriptome data. Among all, tri-repeats were most represented (52%), with the abundance of CCG/CGG repeat motif. A set of 114 polymorphic markers encompassing epigenetic regulators, transcription factors, cell cycle regulators, signaling, and cell wall biogenesis, detected polymorphism and interaction (in silico) with important genes, that might have role in bamboo growth and development. Genetic diversity and population structure of the three D. hamiltonii populations (72 individuals) revealed moderate to high-level genetic diversity (mean alleles per locus: 5.8; mean PIC: 0.44) using neutral EST-SSR markers. AMOVA analysis suggests maximum diversity (59%) exists within population. High genetic differentiation (G(st) = 0.338) and low gene flow (N-m = 0.49) were evident among populations. Further, PCoA, dendrogram, and Bayesian STRUCTURE analysis clustered three populations into two major groups based on geographical separations. In future, SSR marker resources created can be used for systematic breeding and implementation of conservation plans for sustainable utilization of bamboo complex.
机译：Dendrocalamus Hamiltonii是一种巨大的竹种，原产于印度次大陆，经济高度高。尽管如此，十年内高度令人兴奋的自然和开花在传播中施加了严重的限制。遗传多样性基因型的鉴定和混合培养可以协助获得所需性状的成功育种和自然重组。现有遗传多样性和人口结构的表征是有效实施此类策略的必由之可，这面临着由于物种的基于序列标记的不可用而面临重大挑战。在本研究中，从D. Hamiltonii转录组数据中挖掘出8121个EST-SSR标记。其中，三重复最多（52％），具有丰富的CCG / CGG重复基序。一组114个多晶型标志物，包括表观遗传调节剂，转录因子，细胞周期调节剂，信号传导和细胞壁生物发生，检测到具有重要基因的多态性和相互作用（在硅中），这可能在竹生长和发育中具有作用。三D. Hamiltonii人群（72人）的遗传多样性和人口结构揭示了中度至高水平的遗传多样性（每个基因座的平均等位基因：5.8;平均照片：0.44）使用中性EST-SSR标记。 Amova分析表明人口中存在最大多样性（59％）。在群体中，高遗传分化（G（ST）= 0.338）和低基因流动（n-m = 0.49）是显而易见的。此外，PCOA，树枝图和贝叶菊结构分析基于地理分离将三个群体聚集成两个主要群体。未来，创建的SSR标记资源可用于系统的育种和实施竹综合体可持续利用保护计划。

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