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Molecular characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD markers on eggplant.

机译:牡蛎F的分子表征F. Melongenae在茄子上的ISSR和RAPD标记。

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Fusarium oxysporum f. melongenae is a major soil-borne pathogen of eggplant (Solanum melongena). ISSR and RAPD markers were used to characterize Fusarium oxysporum f. melongenae isolates collected from eggplant fields in southern Turkey. Those isolates were not pathogenic to tomato. Pathogens were identified by their morphology, and their identity was confirmed by PCR amplification using the specific primer PF02-3. The isolates were classified into groups on the basis of ISSR and RAPD fingerprints, which showed a level of genetic specificity and diversity not previously identified in Fusarium oxysporum f. melongenae, suggesting that genetic differences are related to the pathogen in the Mediterranean region. The primers selected to characterize Fusarium oxysporum f. melongenae may be used to determine genetic differences and pathogen virulence. This study is the first to characterize eggplant F. oxysporum species using ISSR and RAPD.
机译:Fusarium oxysporum f。 Melongenae是茄子(Solanum Melongena)的主要土壤生物原理。 ISSR和RAPD标记用于表征牡蛎F镰刀菌。 从土耳其南部的茄子领域收集的Melongenae分离物。 那些分离物对番茄不致病。 通过它们的形态鉴定病原体,并通过使用特异性引物PF02-3通过PCR扩增证实它们的身份。 将分离株分类为基于ISSR和RAPD指纹分组,其显示出在Fusarium oxysporum F中未发现的遗传特异性和多样性水平。 Melongenae,旨在遗传差异与地中海地区的病原体有关。 选择用于表征Fusarium Oxysporum F的引物。 Melongenae可用于确定遗传差异和病原体毒力。 本研究是第一个使用ISSR和RAPD表征氧化茄孢菌物种的oxysporum。

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