首页> 外文期刊>Biochemical Genetics >Cloning and characterization of p8 homolog cDNA in the Atlantic halibut (Hippoglossus hippoglossus).
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Cloning and characterization of p8 homolog cDNA in the Atlantic halibut (Hippoglossus hippoglossus).

机译:大西洋大麻(Hippoglossus Hippoglossus)的P8同源物cDNA的克隆与表征。

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摘要

The p8 gene encodes a transcription factor known to modulate cell growth, division, and apoptosis and influences gene expression. In this study, an Atlantic halibut (Hippoglossus hippoglossus) homolog of the p8 gene was cloned, sequenced, and characterized. The full-length p8 cDNA consists of 601 bp and encodes 76 amino acids with a molecular mass of 9 kD. The bHLH region is well conserved between Atlantic halibut and other animals. Analysis by RT-PCR showed that the p8 transcript is constitutively expressed in 9 of the 12 tissues tested: pancreas, intestine, stomach, gill, head kidney, heart, liver, ovary, and spleen. A predicted microRNA target site was found in the 3'UTR of Atlantic halibut p8 mRNA. We speculate that the target site may pair to microRNA molecules because the target site resides in a big loop, a space large enough for the binding of microRNA molecules.
机译:P8基因编码已知调节细胞生长,分裂和细胞凋亡并影响基因表达的转录因子。 在本研究中,克隆,测序和表征了P8基因的大西洋大纤维(Hippoglossus hippoglossus)同源物。 全长P8 cDNA由601bp组成,编码76个氨基酸,分子量为9kd。 BHLH地区在大西洋比较和其他动物之间保守。 RT-PCR分析表明,P8转录物在测试的12个组织中的9个组成型表达:胰腺,肠,胃,鳃,头肾,心脏,肝脏,卵巢和脾脏。 在大西洋大纤维P8 mRNA的3'UTR中发现了预测的MicroRNA靶位点。 我们推测靶位部位可以与MicroRNA分子配对,因为靶位位于大环中,足以足够大的空间,用于微润荷分子的结合。

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