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Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers

机译:RAPD和SRAP标志物从土耳其不同地区收集的仙客来物种的分子表征

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摘要

The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydin, Mugla, Izmir, Denizli, Kahramanmaras, Osmaniye, Eskisehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs. Among the 470 bands generated by the RAPD primers, 467 were polymorphic. The number of bands detected by a single primer set ranged from 11 to 22 (average of 15.6). The percentage polymorphism was 99.3 % based on the RAPD data. In the SRAP analysis, a total of 216 bands were generated, showing 100 % polymorphism. The number of bands detected by a single primer set ranged from 9 to 22 (average of 15.4). All data were scored and UPGMA dendrograms were constructed with similar results in both marker systems, i.e., different species from nine provinces of Turkey were separated from each other in the dendrograms with the same species being clustered together.
机译:Cyclamen(家族Myrsinaceae)含有约20种,其中大部分发生在地中海地区。土耳其具有重点重要的仙客来遗传资源。从土耳其不同地区收集的植物材料的分子表征,其中仙客来生长,即Adaana,Antalya,Aydin,Mugla,Izmir,DeNizli,Kahramanmaras,Osmaniye,使用Rapd和Srap标记进行省份。 DNA通过30个RAPD引物和14个SRAP引物对成功扩增。在RAPD引物产生的470个条带中,467是多态的。由单个底漆集检测的带数范围为11至22(平均为15.6)。基于RAPD数据,多态性百分比为99.3%。在SRAP分析中,产生总共216个带,显示100%多态性。由单个引物集检测的带数范围为9至22(平均为15.4)。所有数据被评分,并且在标记系统中构建了具有类似结果的Upgma树形图,即,在树状图中,来自火鸡的九个省份的不同物种在树状图中分离在一起。

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