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首页> 外文期刊>Biochemical Genetics >Novel Biomarkers for Non-functioning Invasive Pituitary Adenomas were Identified by Using Analysis of microRNAs Expression Profile
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Novel Biomarkers for Non-functioning Invasive Pituitary Adenomas were Identified by Using Analysis of microRNAs Expression Profile

机译：通过使用MicroRNAS表达谱来鉴定用于非功能性侵袭性垂体腺瘤的新型生物标志物

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摘要

The microRNAs (miRNAs) are involved in multiple pathological processes among various types of tumors. However, the functions of miRNAs in benign brain tumors are largely unexplored. In order to explore the pathogenesis of the invasiveness in non-functional pituitary adenoma (NFPA), the miRNAs expression profile was analyzed between invasive and non-invasive non-functional pituitary adenoma by miRNAs microarray. Six most significant differentially expressed miRNAs were identified including four upregulated miRNAs hsa-miR-181b-5p, hsa-miR-181d, hsa-miR-191-3p, and hsa-miR-598 and two downregulated miRNAs hsa-miR-3676-5p and hsa-miR-383. The functions and corresponding signaling pathways of differentially expressed miRNAs were investigated by bioinformatics techniques, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The result of GO analysis indicates regulation of voltage-gated potassium channel activity, positive regulation of sodium ion transport, positive regulation of GTPase activity, negative regulation of Notch signaling pathway, etc. KEGG pathway reveals a series of biological processes, including prolactin signaling pathway, endocrine and other factor-regulated calcium reabsorption, fatty acid metabolism, neuroactive ligand-receptor interaction, etc. The miRNAs hsa-miR-181a-5p was verified by quantitative real-time PCR, and the expression level was in accordance with the microarray result. Our result can provide the evidence on featured miRNAs which play a prominent role in pituitary adenoma as effective biomarkers and therapeutic targets in the future.
机译：MicroRNAS（miRNA）参与各种类型的肿瘤之间的多种病理过程。然而，MiRNA在良性脑肿瘤中的功能在很大程度上是未开发的。为了探讨非功能性垂体腺瘤（NFPA）中侵袭性的发病机制，通过MiRNA微阵列在侵袭性和非侵入性非功能性垂体腺瘤之间分析MiRNA表达谱。鉴定了六种最重要的差异表达的miRNA，包括四种上调的miRNA HSA-miR-181b-5p，hsa-miR-181d，hsa-miR-598和Hsa-miR-598和两个下调的miRNA HSA-miR-3676- 5P和HSA-MIR-383。通过生物信息化技术研究了差异表达的miRNA的功能和相应的信令途径，包括基因本体（GO）和基因组（KEGG）途径分析的基因本体（GO）和京都百科全书。 Go分析结果表明了电压门控钾通道活性的调节，钠离子输送的正调节，GTP酶活性的正调节，Notch信号通路的阴性调节等。Kegg途径揭示了一系列生物过程，包括催乳素信号通路，包括催乳素信号通路，内分泌等因素调节的钙重吸收，脂肪酸代谢，神经活性配体 - 受体相互作用等。通过定量实时PCR验证MiRNA HSA-miR-181A-5P，表达水平符合微阵列结果。我们的结果可以提供关于特色miRNA的证据，它在垂体腺瘤中发挥着突出的作用作为未来的有效生物标志物和治疗目标。

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  • 来源
    《Biochemical Genetics》 |2017年第3期|共15页
  • 作者

    Wu Silin; Gu Ye; Huang Yuying; Wong Tyh-Chai; Ding Hailin; Liu Tengfei; Zhang Yu; Zhang Xiaobiao;

  • 作者单位

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

    Fudan Univ Dept Neurosurg Affiliated Zhongshan Hosp 180 Fenglin Rd Shanghai 200032 Peoples R;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    microRNAs; Expression profile; Invasive pituitary adenoma; Non-functioning pituitary adenoma; Pathogenic mechanism;

    机译：MicroRNA;表达谱;侵入性垂体腺瘤;非功能性垂体腺瘤;病原机制;

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