...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >Comparative Action of Cardiotonic Steroids on Intracellular Processes in Rat Cortical Neurons
【24h】

Comparative Action of Cardiotonic Steroids on Intracellular Processes in Rat Cortical Neurons

机译：枯枝型类固醇对大鼠皮质神经元细胞内方法的比较作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Binding to Na+,K+-ATPase, cardiotonic steroids (CTS) activate intracellular signaling cascades that affect gene expression and regulation of proliferation and apoptosis in cells. Ouabain is the main CTS used for studying these processes. The effects of other CTS on nervous tissue are practically uncharacterized. Previously, we have shown that ouabain affects the activation of mitogen-activated protein kinases (MAP kinases) ERK1/2, p38, and JNK. In this study, we compared the effects of digoxin and bufalin, which belong to different subclasses of CTS, on primary culture of rat cortical cells. We found that CTS toxicity is not directly related to the degree of Na+,K+-ATPase inhibition, and that bufalin and digoxin, like ouabain, are capable of activating ERK1/2 and p38, but with different concentration and time profiles. Unlike bufalin and ouabain, digoxin did not decrease JNK activation after long-term incubation. We concluded that the toxic effect of CTS in concentrations that inhibit less than 80% of Na+,K+-ATPase activity is related to ERK1/2 activation as well as the complex profile of MAP kinase activation. A direct correlation between Na+,K+-ATPase inhibition and the degree of MAP kinase activation is only observed for ERK1/2. The different action of the three CTS on JNK and p38 activation may indicate that it is associated with intracellular signaling cascades triggered by protein-protein interactions between Na+,K+-ATPase and various partner proteins. Activation of MAP kinase pathways by these CTS occurs at concentrations that inhibit Na+,K+-ATPase containing the alpha 1 subunit, suggesting that these signaling cascades are realized via alpha 1. The results show that the signaling processes in neurons caused by CTS can differ not only because of different inhibitory constants for Na+,K+-ATPase.
机译：与Na +，K + -ATP酶，枯枝形类固醇（CTS）的结合激活影响基因表达和细胞增殖和细胞凋亡的细胞内信号传导级联。 Ouabain是用于研究这些过程的主要CTS。其他CTS对神经组织的影响实际上是不表征的。以前，我们已经表明，Ouabain影响了丝裂原激活蛋白激酶（MAP激酶）ERK1 / 2，P38和JNK的激活。在这项研究中，我们比较了Digoxin和Bufalin的效果，它属于CTS的不同亚类，对大鼠皮质细胞的原代培养物。我们发现CTS毒性与Na +，K + -ATPase抑制的程度没有直接相关，并且Bufalin和Digoxin，如Ouabain，能够激活ERK1 / 2和P38，但具有不同的浓度和时间谱。与Bufalin和Ouabain不同，Digoxin在长期孵化后没有降低JNK活化。我们得出结论，CTS在抑制小于80％Na +，K + -AtPase活性的浓度的毒性作用与ERK1 / 2激活以及MAP激酶活化的复杂曲线有关。仅针对ERK1 / 2观察到Na +，K + -ATPase抑制与映射激酶活化程度之间的直接相关性。三个CTS对JNK和P38激活的不同作用可以表明它与在Na +，K + -ATPase和各种伴侣蛋白之间的蛋白质 - 蛋白质相互作用引发的细胞内信号传导级联。这些CTS的MAP激酶途径发生在抑制含有α1亚基的Na +，K + -ATP酶的浓度下，表明这些信号传导级联通过α1实现。结果表明由CTS引起的神经元中的信号传导过程可能不同仅是因为Na +，K + -ATPase的不同抑制常数。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号