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Dynamic characteristics of the mitochondrial genome in SCNT pigs

机译：SCNT猪线粒体基因组的动态特征

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摘要

Most animals generated by somatic cell nuclear transfer (SCNT) are heteroplasmic; inheriting mitochondrial genetics from both donor cells and recipient oocytes. However, the mitochondrial genome and functional mitochondrial gene expression in SCNT animals are rarely studied. Here, we report the production of SCNT pigs to study introduction, segregation, persistence and heritability of mitochondrial DNA transfer during the SCNT process. Porcine embryonic fibroblast cells from male and female Xiang pigs were transferred into enucleated oocytes from Yorkshire or Landrace pigs. Ear biopsies and blood samples from SCNT-derived pigs were analyzed to characterize the mitochondrial genome haplotypes and the degree of mtDNA heteroplasmy. Presence of nuclear donor mtDNA was less than 5% or undetectable in ear biopsies and blood samples in the majority of SCNT-derived pigs. Yet, nuclear donor mtDNA abundance in 14 tissues in F0 boars was as high as 95%. Additionally, mtDNA haplotypes influenced mitochondrial respiration capacity in F0 fibroblast cells. Our results indicate that the haplotypes of recipient oocyte mtDNA can influence mitochondrial function. This leads us to hypothesize that subtle developmental influences from SCNT-derived heteroplasmy can be targeted when using donor and recipient mitochondrial populations from breeds of swine with limited evolutionary divergence.
机译：大多数由体细胞核转移（SCNT）产生的动物是异质的;从供体细胞和受体卵母细胞中遗传线粒体遗传学。然而，很少研究SCNT动物中的线粒体基因组和功能性线粒体基因表达。在这里，我们报告了SCNT猪的生产，研究了SCNT过程中线粒体DNA转移的引入，分离，持续性和遗传性。来自雄性和雌性Xiang猪的猪胚胎成纤维细胞从约克夏或兰德斯猪转移到Enucleated卵母细胞中。分析来自SCNT衍生猪的耳朵活组织检查和血液样品，以表征线粒体基因组单倍型和MTDNA异质的程度。核供体MTDNA的存在小于耳朵活组织检查和大多数SCNT衍生的猪的血液样本中的5％或不可检测到的。然而，F0猪中14个组织中的核供体MTDNA丰度高达95％。另外，MTDNA单倍型影响F0成纤维细胞中的线粒体呼吸能力。我们的结果表明，受体卵母细胞MTDNA的单倍型可以影响线粒体功能。这导致我们假设来自SCNT衍生的异质的微妙发育影响可以靶向，当使用具有有限的进化发散的猪的猪的供体和受体线粒体种群时可以靶向。

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  • 来源
    《Biological chemistry》 |2019年第5期|共11页
  • 作者

    TaoYin; JikunWang; HaiXiang; Carl A.Pinkert; QiuyanLi; XingboZhao;

  • 作者单位

    College of Animal Science and Technology China Agricultural University 100193 Beijing China;

    Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization;

    School of Life Science and Engineering Foshan University Foshan 528225 China;

    Department of Biological Sciences College of Arts and Sciences The University of Alabama;

    State Key Laboratory for Agribiotechnology College of Biological Sciences China Agricultural;

    College of Animal Science and Technology China Agricultural University 100193 Beijing China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    heteroplasmy; mitochondria; mtDNA; SCNT pigs;

    机译：异质;线粒体;mtdna;scnt猪;

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