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首页> 外文期刊>Cytokine >Effects of hypothermia and re-warming on the inflammatory response in a murine multiple hit model of trauma.
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Effects of hypothermia and re-warming on the inflammatory response in a murine multiple hit model of trauma.

机译：低温和复温对小鼠多发性外伤模型的炎症反应的影响。

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INTRODUCTION: Although, hypothermia is a frequent event after trauma, it is unclear whether its beneficial or detrimental effects are more important. This study aims to quantify the effects of hypothermia and re-warming on the inflammatory response after fracture/hemorrhage and subsequent fracture stabilization with resuscitation. MATERIALS AND METHODS: Eighty-one male C57Bl/6 mice (aged 8-10 weeks, weighing 22.0+/-3.0g) underwent femoral fracture and hemorrhage followed by resuscitation and splint fixation of the fracture. Animals were sacrificed 3h after induction of hemorrhage and fracture. Besides a sham group (n=6), four experimental groups were created: A: normothermia (n=12), B: hypothermia after trauma (n=21), C: re-warming after resuscitation and before stabilization (n=21), and D: hypothermia before trauma (n=21). Groups B-D were further subdivided into three subgroups according to the degree of hypothermia (subgroup 1: 35-33 degrees C, subgroup 2: 32.9-30.0 degrees C, and subgroup 3: 29.9-27.0 degrees C). Plasma cytokine (TNF-alpha, IL-6, and IL-10) and chemokine (MCP-1) concentrations were determined by ELISA, pulmonary permeability changes were quantified, and histological analysis of lung and liver tissues was performed. RESULTS: Normothermia resulted in a significantly increased early mortality rate. A significantly increased pro-inflammatory and decreased anti-inflammatory responses were also observed in normothermia as compared to hypothermia. The extent of these changes was most pronounced in the severe hypothermic group. Re-warming after mild hypothermia resulted in a pro-inflammatory response comparable to normothermia. CONCLUSION: Hypothermia has a beneficial effect on early survival after trauma, which appears to be independent of the level of hypothermia and re-warming. Re-warming, however, enhanced the pro-inflammatory response. Further studies with a longer posttraumatic observation period are required to investigate the long term effects of the hypothermia and re-warming-induced changes on the pro- and anti-inflammatory responses.
机译：简介：尽管体温过低是创伤后的常见事件，但尚不清楚其有益还是有害作用更为重要。这项研究的目的是量化体温过低和再升温对骨折/出血后的炎症反应以及随后的复苏后骨折稳定的影响。材料与方法：八十一只雄性C57Bl / 6小鼠（年龄8-10周，体重22.0 +/- 3.0g）经历了股骨骨折和大出血，然后进行了复苏和夹板固定骨折。诱导出血和骨折后3小时处死动物。除假手术组（n = 6）外，还创建了四个实验组：A：正常体温（n = 12），B：创伤后体温过低（n = 21），C：复苏后和稳定前复温（n = 21） ），D：创伤前的体温过低（n = 21）。根据低温程度，将B-D组进一步分为三个子组（子组1：35-33摄氏度，子组2：32.9-30.0摄氏度，子组3：29.9-27.0摄氏度）。通过ELISA测定血浆细胞因子（TNF-α，IL-6和IL-10）和趋化因子（MCP-1）的浓度，定量肺通透性变化，并对肺和肝组织进行组织学分析。结果：正常体温导致早期死亡率显着增加。与体温过低相比，在正常体温中还观察到促炎反应显着增加和抗炎反应减少。这些变化的程度在严重的低温组中最为明显。轻度体温过低后再次变暖可导致与正常体温相当的促炎反应。结论：体温过低对创伤后的早期生存具有有益的作用，这似乎与体温过低和再升温无关。然而，重新变暖增强了促炎反应。创伤后观察期较长的进一步研究需要研究体温过低和再升温引起的变化对促炎和消炎反应的长期影响。

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