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首页> 外文期刊>Cytokine >The effect of MCP-1 depletion on chemokine and chemokine-related gene expression: evidence for a complex network in acute inflammation.
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The effect of MCP-1 depletion on chemokine and chemokine-related gene expression: evidence for a complex network in acute inflammation.

机译：MCP-1耗竭对趋化因子和趋化因子相关基因表达的影响：急性炎症中复杂网络的证据。

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摘要

The expression of chemokines has been suggested to involve an interdependent network, with the absence of a single chemokine affecting the expression of multiple other chemokines. Monocyte chemoattractant protein (MCP-1), a member of C-C chemokine superfamily, plays a critical role in the recruitment and activation of leukocytes during acute inflammation. To examine the effect of the loss of MCP-1 on expression of the chemokine network, we compared the mRNA expression profiles of MCP-1(-/-) and wild type mice during the acute inflammatory phase of excisional wounds. Utilizing a mouse cDNA array containing 514 chemokine and chemokine related genes, the loss of MCP-1 was observed to cause a significant upregulation of nine genes (Decorin, Persephin, IL-1beta, MIP-2, MSP, IL1ra, CCR5, CCR3, IL-11) and significant downregulation of two genes (CCR4 and CD3Z) in acute wounds. The array data was confirmed by semi-quantitative RT-PCR. The effect of MCP-1 deletion on chemokine expression was further examined inisolated macrophages. Compared to wild type, LPS-stimulated peritoneal macrophages from MCP-1(-/-) mice showed a significant increase in the expression of RANTES, MIP-1beta, MIP-1alpha and MIP-2 mRNA. The data suggest that loss of a single chemokine perturbs the chemokine network not only in the setting of acute inflammation but even in an isolated inflammatory cell, the macrophage.
机译：已经提出趋化因子的表达涉及相互依赖的网络，没有单个趋化因子的存在会影响多种其他趋化因子的表达。单核细胞趋化蛋白（MCP-1）是C-C趋化因子超家族的成员，在急性炎症过程中对白细胞的募集和激活起关键作用。为了检查MCP-1丢失对趋化因子网络表达的影响，我们比较了切除伤口急性炎症期MCP-1（-/-）和野生型小鼠的mRNA表达谱。利用包含514个趋化因子和趋化因子相关基因的小鼠cDNA阵列，观察到MCP-1的缺失导致9个基因（Decorin，Persephin，IL-1beta，MIP-2，MSP，IL1ra，CCR5，CCR3， IL-11）和两个基因（CCR4和CD3Z）在急性伤口中的显着下调。阵列数据通过半定量RT-PCR确认。 MCP-1删除对趋化因子表达的影响进一步检查了孤立的巨噬细胞。与野生型相比，来自MCP-1（-/-）小鼠的LPS刺激的腹膜巨噬细胞显示RANTES，MIP-1beta，MIP-1alpha和MIP-2 mRNA的表达显着增加。数据表明，单个趋化因子的丧失不仅在急性炎症的环境中，甚至在孤立的炎性细胞巨噬细胞中也扰乱了趋化因子网络。

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