...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica, Section F. Structural biology and crystallization communications >Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans
【24h】

Purification, crystallization and preliminary X-ray analysis of adenylosuccinate synthetase from the fungal pathogen Cryptococcus neoformans

机译：真菌病原体新隐球菌腺苷琥珀酸合成酶的纯化，结晶和初步X射线分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

With increasingly large immunocompromised populations around the world, opportunistic fungal pathogens such as Cryptococcus neoformans are a growing cause of morbidity and mortality. To combat the paucity of antifungal compounds, new drug targets must be investigated. Adenylosuccinate synthetase is a crucial enzyme in the ATP de novo biosynthetic pathway, catalyzing the formation of adenylosuccinate from inosine monophosphate and aspartate. Although the enzyme is ubiquitous and well characterized in other kingdoms, no crystallographic studies on the fungal protein have been performed. Presented here are the expression, purification, crystallization and initial crystallographic analyses of cryptococcal adenylosuccinate synthetase. The crystals had the symmetry of space group P2(1)2(1)2(1) and diffracted to 2.2 angstrom resolution.
机译：随着世界范围内越来越多的免疫功能低下的人群，诸如新隐球菌的机会性真菌病原体成为发病率和死亡率日益增长的原因。为了消除抗真菌化合物的不足，必须研究新的药物靶标。腺苷琥珀酸酯合成酶是ATP从头生物合成途径中的关键酶，催化肌苷单磷酸酯和天冬氨酸形成腺苷琥珀酸酯。尽管该酶无处不在并且在其他王国中具有很好的特征，但尚未对真菌蛋白进行结晶学研究。这里介绍的是隐球菌腺苷琥珀酸酯合成酶的表达，纯化，结晶和初步晶体学分析。晶体具有空间群P2（1）2（1）2（1）的对称性，并衍射至2.2埃分辨率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号