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首页> 外文期刊>Biomaterials Science >The surrounding tissue contributes to smooth muscle cells' regeneration and vascularization of small diameter vascular grafts
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The surrounding tissue contributes to smooth muscle cells' regeneration and vascularization of small diameter vascular grafts

机译：周围组织有助于平滑肌肉细胞的再生和小直径血管移植物的血管形成

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摘要

Small diameter vascular grafts have been promising substitutes for bypass surgery to treat cardiovascular disease. However, no ideal product is available in the clinic. In order to design improved, next generation vascular grafts, it is essential to understand the cellular and molecular mechanisms underlying tissue regeneration after vascular graft implantation. Two diverse microenvironments, circulating blood and the surrounding tissue, are involved in the regeneration process after vascular graft implantation in situ. However, their regenerative functions are not completely understood. To elucidate their roles in regeneration, we used electrospinning to fabricate four types of tubular scaffolds with a structure consisting of a microfiber layer (fiber diameter approximate to 6 m) and a nanofiber layer (fiber diameter < 1 m): microfiber scaffold, nanofiber scaffold, outer microfiber bilayer scaffold and inner microfiber bilayer scaffold. In the outer microfiber scaffold, cells from the surrounding tissue were allowed into the scaffold but not cells from the circulating blood while it was opposite in the inner microfiber scaffold. The processes of endothelium formation, smooth muscle cell regeneration, neo-tissue formation and vascularization of these scaffolds were analyzed with a rat left common carotid artery replacement model. Our data showed that smooth muscle cells' regeneration and vascularization were different among the four types of scaffolds. The thickest neo-tissue and -SMA(+) cell layers were detected in the microfiber scaffold group while the thinnest in the nanofiber scaffold group, and thicker neo-tissue and -SMA(+) cell layers were found in the outer microfiber bilayer scaffold group compared to the inner microfiber bilayer scaffold group. In addition, vascularization in the outer microfiber bilayer scaffold group and microfiber group was dramatically better than the inner microfiber bilayer scaffold group and the nanofiber group. Furthermore, we demonstrated that the regenerated SMCs were associated with the CD206(+) macrophages in the graft wall. In all, the microfiber scaffold showed the best neo-tissue regeneration in vivo. These results indicate that the surrounding tissue contributes more to vascular regeneration than circulating blood. This finding gives a significant design clue that modulating the vascular surrounding tissue will be an alternative strategy for designing advanced and feasible small diameter vascular grafts.
机译：小直径血管移植是旁路手术的替代品，以治疗心血管疾病。但是，诊所没有提供理想的产品。为了设计改进，下一代血管移植物，必须了解血管移植物注入后基础组织再生的细胞和分子机制。两种不同的微环境，循环血液和周围组织，在原位血管移植物植入后参与再生过程。然而，它们的再生功能并不完全理解。为了阐明其在再生中的作用，我们使用静电纺丝来制造四种类型的管状支架，其具有由微纤维层（纤维直径近似至6μm）和纳米纤维层（纤维直径<1米）的结构组成：微纤维支架，纳米纤维支架，外微纤维双层脚手架和内微纤维双层脚手架。在外部微纤维支架中，将来自周围组织的细胞允许进入支架，但在内微纤维支架中与循环血液中没有细胞。用大鼠留下常见的颈动脉替代模型，分析了这些支架的内皮形成，平滑肌细胞再生，新组织形成和血管化的过程。我们的数据显示，光滑的肌肉细胞的再生和血管化在四种类型的支架中不同。在微纤维支架组中检测到最厚的新组织和-Sma（+）细胞层，同时在外部微纤维双层支架中发现纳米纤维支架基团中最薄，较厚的新组织和-Sma（+）细胞层组与内微纤维双层支架组相比。此外，外部微纤维双层支架组和微纤维组中的血管化比内微纤维双层支架基团和纳米纤维组显着更好。此外，我们证明再生的SMC与移植物壁中的CD206（+）巨噬细胞有关。总而言之，微纤维支架显示体内最佳的新组织再生。这些结果表明，周围组织促进血管再生的血管再生多于循环血液。该发现提供了一种重要的设计线索，其调节血管周围组织将是设计先进和可行的小直径血管移植物的替代策略。

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  • 来源
    《Biomaterials Science》 |2019年第3期|共12页
  • 作者

    Liu Jing; Qin Yibo; Wu Yifan; Sun Zhiting; Li Binhan; Jing Huimin; Zhang Chuangnian; Li Chen; Leng Xigang; Wang Zhihong; Kong Deling;

  • 作者单位

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Nankai Univ Coll Life Sci State Key Lab Med Chem Biol Key Lab Bioact Mat Minist Educ Tianjin 300071 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

    Chinese Acad Med Sci Tianjin Key Lab Biomat Res Inst Biomed Engn Tianjin 300192 Peoples R China;

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  • 正文语种 eng
  • 中图分类 分子生物学;
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